Sample processing plan for fatty acid determination in food

GB28050-2025 National Food Safety Standard "General Principles for Nutrition Labeling of Prepackaged Foods" was released on March 16, 2025. The mandatory labeling content in the nutrition table has been added to "1+6" on the basis of the original "1+4", which means that saturated fats (acids) and sugars have been added on the basis of energy, protein, fat, carbohydrates, and sodium. Beijing Aowei Instrument Co., Ltd. has developed a step-by-step process for the hydrolysis, fat extraction, ether layer evaporation, saponification and methylation of samples based on GB5009.168-2016 "Method for determination of fatty acid content in food"Sample processing plan for fatty acid determination in food

Sample hydrolysis unit:

FH32 high-throughput hydrolysis device can perform acid and alkali hydrolysis treatment on 32 samples simultaneously. The innovative gentle stirring technology avoids losses caused by sample wall sticking;

Fat extraction unit:

After the hydrolysis of the sample is completed, there is no need to change the container, and the double ether is directly added for fat extraction in the LL16 high-throughput fat extractor. It can process 16 samples simultaneously without the need for pressure relief and gas release during the extraction process;

Ether layer evaporation unit:

By using a direct pouring method and combining the ether layers into a dedicated evaporation bottle, the APE16 vacuum parallel concentrator can rapidly evaporate 16 samples;

Saponification methyl esterification unit:

The ether layer evaporation is complete, and the sample does not need to be transferred. The corresponding reagents are directly added to the evaporation bottle and subjected to saponification and methylation treatment on AF16. AF16 can process 16 samples simultaneously and has independent automatic lifting function. During use, there is no need to build an iron frame to fix the glass components;