SEMG1 ELISA Kit

Product Introduction: SEMG1 ELISA Kit

: 96T/box, 48T/box,

[Brand]: Nalian Biology

: ELISA reader

【 Samples to be tested 】: Cell culture supernatant, tissue homogenate, body fluids, urine, serum plasma, cerebrospinal fluid, pleural and peritoneal fluid specimens, etc

Complete species: Human, Rat, Mouse, Rabbit, Dog, Chicken, Pig, Plant ELISA kits, etc

【 Specificity 】: This ELISA detection kit can simultaneously detect natural or recombinant proteins without cross reactivity with other related proteins

[Scope of Application]: This kit is only used for scientific research and is prohibited from being used in clinical practice

Detection principle: The reagent kit adopts a double antibody one-step sandwich enzyme-linked immunosorbent assay (ELISA). Add the sample, standard, and HRP labeled detection antibody in sequence to the pre coated micropores of mitochondrial respiratory chain complex II (COX II) antibody, incubate and wash. Using substrate TMB for color development, TMB is converted to blue under the catalysis of peroxidase and to the final yellow under the action of acid. The depth of color is positively correlated with mitochondrial respiratory chain complex II (COX II) in the sample. Measure the absorbance (OD value) at 450nm wavelength using an enzyme-linked immunosorbent assay (ELISA) reader and calculate the sample activity.

SEMG1 ELISA KitComposition: (Please refer to the instruction manual for the detection range)

Precautions for operation:

1. The reagent kit should be stored at 2-8 ℃ and equilibrated at room temperature for 20 minutes before use. The concentrated washing solution taken out of the refrigerator will have crystals, which is a normal phenomenon. Heating in a water bath will dissolve the crystals before use.

2. The Flat noodles not used in the experiment shall be immediately put back into the self sealing bag and sealed (low-temperature dry) for storage.

Standard S0 with 0 activity can be considered as a negative control or blank; When operating according to the instructions, the sample has already been diluted 5 times, and the actual activity of the sample is determined by multiplying the final result by 5.

4. Strictly follow the time, liquid dosage, and sequence indicated in the instructions for incubation operation.

5. Shake all liquid components thoroughly before use.

Advantages of Nalian Biological ELISA Detection Kit:

Advantage 1: can cover multiple species such as humans, rats, mice, guinea pigs, rabbits, pigs, horses, dogs, and cats, with complete detection indicators such as cytokine detection, myocardial infarction detection, endocrine detection, liver fiber detection, etc.

Advantage 2: It can detect various types of specimens such as serum, plasma, various tissue samples, cell culture supernatant, urine, cerebrospinal fluid, feces, etc.

Advantage 3: [Quality and Assurance] All of our products undergo quality inspection before being shipped out, and can only be shipped after passing the inspection, ensuring that the product quality is qualified and allowing you to use it with peace of mind. If there are any quality issues with our company, we will immediately provide a free return and refund!

Advantage 4: After years of research and development experience, debugging, and correction by technical personnel, the experimental effect of the reagent kit is stable and the detection time is short (2-3 hours can be completed), with high sensitivity, high repeatability, good adsorption performance, and affordable price.