SARS-CoV-2 nucleocapsid protein infectious disease antigen

Product Name: SARS-CoV-2 Nucleocapsid Protein (His Tag) (HPLC verified)
Product Code: HPLC-40588-V07E
Specification: 100 μ g/tube
As a professional protein antibody supplier, Chongqing Mores Biotech provides you with spot supplySARS-CoV-2 nucleocapsid protein infectious disease antigenWelcome to consult

core parameters

Source: SARS-CoV-2 wild-type
Login number: YP_009724397.2
Expression region: Met1 – Ala419 (N-terminus of full-length nucleocapsid protein)
Host expression: Escherichia coli (E. coli)
Tag: N-terminal His tag (polymeric amino acid tag)
Amino acid length: 426 aa (including tag)
Theoretical molecular weight: 46.59 kDa
Purity:
SDS-PAGE：≥95%
SEC-HPLC: ≥ 95% (rigorously validated by HPLC)
Purity verification: HPLC (SEC-HPLC)+SDS-PAGE dual quality control
Endotoxin:<1 EU/μ g
Buffer solution: PBS, pH 7.4
Storage: -20 ℃ to -80 ℃, avoid repeated freezing and thawing

Product Overview
HPLC-40588-V07E is a high purity nucleocapsid protein (N protein) of new coronavirus. It has been strictly verified by SEC-HPLC that its purity is more than 95%, oligomer and low endotoxin. It is specially designed for antigen detection, antibody development, serological detection, vaccine quality control, ELISA/CLIA standard products and other high sensitivity experiments.

N protein: COVID-19 · virus is rich, highly conservative, strong immunogenic structural protein, which is the core target of antigen/antibody detection.
HPLC grade: higher purity, fewer aggregates, and more stable batch compared to ordinary grade (40588-V07E).
His tag: convenient for purification, fixation, coupling, and WB tracing.

Main applications

Serological testing: ELISA/CLAI/colloidal gold antigen/antibody detection kit for antigen detection
Antibody development: monoclonal antibody/multi antibody/neutralizing antibody immunogenicity, screening, epitope identification
Vaccine quality control: evaluation of vaccine efficacy, stability, and cross reactivity
Protein interaction: N-protein RNA, N-protein host protein binding experiments
Standard products: antigen quantification, methodological establishment, quality control comparison
Diagnostic material: IVD antigen detection core material

Product Advantages

HPLC dual quality control, ultra-high purity oligomer SEC-HPLC validation>95% monomer, no polymer, avoiding non-specific binding and background interference.
Highly conserved sequence, covering the wild-type N protein of mainstream variants and highly cross reactive variants such as Alpha/Beta/Gamma/Delta/Omron.
High immunogenicity, suitable for antibody/diagnostic development with natural conformation and strong antigenicity, suitable for immunization, coating, and paired screening.
Low endotoxin, no animal source, batch stable endotoxin<1 EU/μ g, no serum/pancreatic enzyme/BSA, strong experimental consistency.

Common FAQ
Q: Is it different from the regular 40588-V07E? A: HPLC-40588-V07E is a high-purity HPLC validation grade with monomer purity>95% and fewer aggregates, suitable for IVD and high-sensitivity experiments.
Q: Which testing platforms are applicable? A：ELISA、CLIA、Western Blot、 Immunofluorescence, colloidal gold, BLI/SPR, etc.
Q: Recommended package concentration? A: ELISA commonly uses 0.1-1 μ g/mL; Colloidal gold/CLIA optimized by platform.
Q: Can it be used for vaccine quality control? A: Suitable for evaluating vaccine antigen content, immunogenicity, and cross reactivity.
As a professional protein antibody supplier, Chongqing Mores Biotech provides you with spot supplySARS-CoV-2 nucleocapsid protein infectious disease antigenWelcome to consult