Research antigen supplier

Product Name:Research antigen supplier
English name:
Specification: 50T
Storage conditions: Store at -20 ℃ away from light and avoid repeated freezing and thawing.
Transportation: Low temperature, dark, free home delivery by express delivery.
Product Features:
◇ High specificity: No cross reactivity with other viruses, no non-specific amplification;
◇ High sensitivity: The detection sensitivity can reach 10-100 copies;
◇ Easy to operate: All reagents in this series use the same system and conditions, and can perform multiple tests simultaneously;
◇ High throughput: Multiple dual PCR detection and triple PCR detection kits.
Prepare items:
Cleaning solution (A) milliliters
Staining solution (t B) microliters
Dilute solution (C) milliliters
Dissolved solution (tD) milliliters
1 copy of product manual

The specific determining factors of PCR reaction are:
① Primers bind specifically and correctly to template DNA;
② Principle of base pairing;
③ The fidelity of Taq DNA polymerase synthesis reaction;
④ The specificity and conservation of target genes.
The correct binding of primers and templates is crucial. The binding of primers to templates and the extension of primer chains follow the principle of base pairing. The fidelity of polymerase synthesis reaction and the high temperature resistance of Taq DNA polymerase enable the binding (refolding) of template and primer in the reaction to occur at higher temperatures, greatly increasing the specificity of binding and maintaining high accuracy of amplified target gene fragments. By selecting target gene regions with high specificity and conservation, the degree of specificity is even higher.
(2) High sensitivity
The production of PCR products increases exponentially and can amplify the initial test template at the picogram (pg=10-12 g) level to the microgram (ug=10-6 g) level. Can detect one target cell from one million cells; In virus detection, the sensitivity of PCR can reach 3 RFUs (Spot Formation Units); The lowest detection rate in bacteriology is 3 bacteria.
(3) Simple and fast
The PCR reaction uses high-temperature resistant Taq DNA polymerase. After adding the reaction solution in one go, the denaturation annealing extension reaction is carried out on the DNA amplification solution and water bath. The amplification reaction is usually completed within 2-4 hours. The amplification products are generally analyzed by electrophoresis and do not necessarily require isotopes. They are free from radioactive contamination and easy to promote.
(4) Low purity requirements for specimens
There is no need to isolate viruses or bacteria or cultivate cells. Both crude DNA and total RNA can be used as amplification templates. Clinical specimens such as blood, bodily fluids, mouthwash, hair, cells, and live PCR techniques can be directly used.
Research resistanceoriginalNotes:
① The order of adding reagents should be * to ensure that the incubation time for all reaction plate wells is the same.
② Use clean plastic containers to prepare detergent.
③ Incubate according to the time, amount, and sequence indicated in the instructions of the chicken hemoglobin (HB) ELISA detection kit.
④ Substrate A should evaporate and avoid opening the lid for a long time. Substrate B is sensitive to light and should be avoided from prolonged exposure to light. Avoid contact with hands, toxic. After the experiment is completed, the OD value should be read immediately.
⑤ When washing the enzyme-linked immunosorbent assay (ELISA) plate, it should be thoroughly dried. Do not directly put the absorbent paper into the ELISA reaction well to absorb water.
⑥ Use disposable suction tips to avoid cross contamination, and avoid using sample dispensers with metal parts when suctioning termination solution and substrates A and B.
⑦ The Flat noodles not used in the experiment shall be immediately put back into the packaging bag and sealed for storage to avoid deterioration.
⑧ Reagents should be stored according to the label instructions and returned to room temperature before use. The diluted standard should be discarded and cannot be stored.
⑨ Other unused reagents should be packaged or covered. Do not mix reagents of different batches. Use before shelf life.

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Five elements of reaction:
There are five main substances involved in PCR reactions, namely primers, enzymes dNTP、 Template and Mg2+
Primers: Primers are crucial for PCR specific reactions, and the specificity of PCR products depends on the degree of complementarity between primers and template DNA. In theory, as long as any template DNA sequence is known, complementary oligonucleotide chains can be designed as primers, and PCR can be used to amplify the template DNA in large quantities in vitro. The design of primers should follow the following principles:
① Primer length: 15-30bp, commonly around 20bp.
② Primer amplification span: 200-500bp is suitable, and under specific conditions, the fragment can be expanded to a length of 10kb.
③ Primer base: A G+C content of 40-60% is recommended. Insufficient G+C results in poor amplification, while excessive G+C can lead to non-specific bands. ATGC machine distribution, avoiding the string arrangement of more than 5 purine or pyrimidine nucleotides.
④ Avoid secondary structures within primers and avoid complementarity between two primers, especially at the 3 'end, as this can result in primer dimers and non-specific amplification bands.
⑤ The base at the 3 'end of the primer, especially the last and second to last base, should be strictly paired to avoid PCR failure due to mismatched end bases.
⑥ Primers may have or can add suitable enzyme cleavage sites, and the amplified target sequence has suitable enzyme cleavage sites, which is beneficial for enzyme cleavage analysis or molecular cloning.
⑦ Primer specificity: Primers should have no apparent homology with other sequences in the nucleic acid sequence database.
Primer quantity: The concentration of each primer is 0.1-1umol or 10-100pmol. Low primer quantity is better to produce the desired results. High primer concentration can cause mismatches and non-specific amplification, and can increase the chance of dimer formation between primers.