Rat lactate dehydrogenase (LDH) ELISA kit

Detecting species

People, mice, rats, rabbits, pigs, cows, sheep, chickens, ducks, fish, horses, plants, etc

Detecting targets

Inflammatory factors（IL-6, TNF - α, IFN - γ, etc.), hormones, biomarkers (CRP, VEGF, etc.), signaling pathway proteins, apoptosis markers, cardiovascular markers, etc

detection method

Double antibody one-step sandwich method, competitive method, indirect method

Product Specifications

48T/box, 96T/box

Sample Type

Serum, plasma, cell culture supernatant, tissue homogenate, urine, pleural and peritoneal fluid, cerebrospinal fluid, lavage fluid, etc

1. Serum: Place the whole blood sample collected in the serum separation tube at room temperature for 2 hours or overnight at 4 ℃, then centrifuge at 1000 × g for 20 minutes to obtain the supernatant, or store the supernatant at -20 ℃ or -80 ℃, but avoid repeated freezing and thawing.

2. Plasma: Collect specimens using EDTA or heparin as anticoagulants, and centrifuge the specimens at 2-8 ℃ 1000 × g for 15 minutes within 30 minutes after collection. The supernatant can be collected for detection, or stored at -20 ℃ or -80 ℃, but repeated freezing and thawing should be avoided.

3. Tissue homogenate: Rinse the tissue with pre cooled PBS (0.01M, pH=7.4) to remove residual blood (lysed red blood cells in the homogenate can affect measurement results), weigh and crush the tissue. Mix the shredded tissue with the corresponding volume of PBS (usually in a weight to volume ratio of 1:9, for example, 1g of tissue sample corresponds to 9mL of PBS, the specific volume can be adjusted appropriately according to experimental needs, and records should be kept. Recommend adding protease inhibitors to PBS and grinding thoroughly on ice in a glass homogenizer. To further lyse tissue cells, the homogenate can be sonicated or subjected to repeated freeze-thaw cycles. Finally, centrifuge the homogenate at 5000 × g for 5-10 minutes and collect the supernatant for detection.

4. Cell culture supernatant: Centrifuge at 1000 × g for 20 minutes, take the supernatant for detection, or store the supernatant at -20 ℃ or -80 ℃, but avoid repeated freezing and thawing.

5. Other biological specimens: Centrifuge at 1000 × g for 20 minutes and collect the supernatant for detection.

6. Sample appearance: The sample should be clear and transparent, and suspended solids should be removed by centrifugation.

7. Sample storage: If the sample is collected and tested within one week, it can be stored at 4 ℃. If it cannot be tested in a timely manner, it should be packaged according to a single use amount and frozen at -20 ℃ (tested within one month) or -80 ℃ (tested within six months) to avoid repeated freezing and thawing. Hemolysis of the sample will affect the final test results, so hemolytic samples should not be tested for this purpose.

8. Serum: Place the whole blood sample collected in the serum separation tube at room temperature for 2 hours or overnight at 4 ℃, then centrifuge at 1000 × g for 20 minutes to obtain the supernatant, or store the supernatant at -20 ℃ or -80 ℃, but avoid repeated freezing and thawing.

9. Plasma: Collect specimens using EDTA or heparin as anticoagulants, and centrifuge the specimens at 2-8 ℃ 1000 × g for 15 minutes within 30 minutes after collection. The supernatant can be collected for detection, or stored at -20 ℃ or -80 ℃, but repeated freezing and thawing should be avoided.

10. Tissue homogenate: Rinse the tissue with pre cooled PBS (0.01M, pH=7.4) to remove residual blood (lysed red blood cells in the homogenate can affect measurement results), weigh and crush the tissue. Mix the shredded tissue with the corresponding volume of PBS (usually in a weight to volume ratio of 1:9, for example, 1g of tissue sample corresponds to 9mL of PBS, the specific volume can be adjusted appropriately according to experimental needs, and records should be kept. Recommend adding protease inhibitors to PBS and grinding thoroughly on ice in a glass homogenizer. To further lyse tissue cells, the homogenate can be sonicated or subjected to repeated freeze-thaw cycles. Finally, centrifuge the homogenate at 5000 × g for 5-10 minutes and collect the supernatant for detection.

11. Cell culture supernatant: Centrifuge at 1000 × g for 20 minutes to obtain the supernatant for detection, or store the supernatant at -20 ℃ or -80 ℃, but avoid repeated freezing and thawing.

12. Other biological specimens: Centrifuge at 1000 × g for 20 minutes and collect the supernatant for detection.