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Address
No. 16 Guizhou Road, Heping District, Tianjin
Tianjin Peptide Chain Biotechnology Co., Ltd
No. 16 Guizhou Road, Heping District, Tianjin
Rabbit interleukin-17 (IL-17) rabbit ELISA kit
Sample collection, processing, and preservation methods
1. Serum: Use test tubes free of pyrogens and endotoxins, avoid any cell irritation during the operation, collect blood, centrifuge at 3000 rpm for 10 minutes, and quickly and carefully separate serum and red blood cells.
2. Plasma: anticoagulant with EDTA, citrate or heparin. Centrifuge at 3000 rpm for 30 minutes and collect the supernatant.
3. Cell supernatant: Centrifuge at 3000 rpm for 10 minutes to remove particles and polymers.
4. Tissue homogenate: Crush the tissue by adding an appropriate amount of physiological saline. Centrifuge at 3000 rpm for 10 minutes and collect the supernatant.
5. Storage: If the sample is not tested in a timely manner after collection, please divide it into batches according to a single dose, freeze it at -20 ℃, avoid repeated freezing and thawing, thaw it at room temperature, and ensure that the sample is evenly filled and thawed.
Personal belongings
1. ELISA reader(450nm)
2. High precision sampler and gun head:0.5-10uL、2-20uL、20-200uL、200-1000uL
3. 37 ℃ constant temperature box
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