RK1 cell specific culture medium instruction manual

Transportation and storage:

The company's products are only used for scientific researchDepending on the weather conditions and transportation distance, the company and the customer will negotiate and choose one of the following methods.

1) 1mL cryopreserved cell suspension is put into 1.8ml cryopreserved tube and transported in foam insulation box filled with dry ice; After receiving the cells, please thaw and revive them for cultivation as soon as possible. If resuscitation cannot be performed immediately, the frozen cells can be stored at -80 ℃ for one month.

2) The T-25 culture bottle is filled with culture medium and transported at room temperature; After receiving the cells, please observe the growth status of the cells under a microscope. If the bottle laying rate exceeds 85%, immediately perform passaging operation. If there are many suspended cells, please place the culture bottle in the incubator overnight to help non dead suspended cells adhere to the wall again.

Specification parameters:

Carefully optimized by the technical team and tested for a long time, this product can maintainRK1 cells are in a good growth state. This product already contains various ingredients required for the growth of RK1 cells, without the need to add any ingredients, and can be directly used for in vitro culture of RK1 cells. This product is for further scientific research use only and should not be used for diagnosis, treatment, clinical, household, or other purposes.

Product Description

product form: Liquid

product concentration:1×

Product Specifications:125mL×4

culture medium composition:RPMI-1640＋15% FBS＋1% P/S

Bacterial detection: Negative

fungal detection: Negative

Mycoplasma testing: Negative

Cell Growth ExperimentGood cell growth and normal morphology

Endotoxin content(EU/mL):≤3

Storage conditions: 2-8 ℃, stored away from light

transport conditionsIce bag refrigerated transportation

Validity period: 3 months

How to handle received:

1. Firstly, observe whether the culture bottle is intact and whether there is any leakage or turbidity in the culture medium. If there are any, please take photos and contact technical support in a timely manner (the photos taken will be used as the basis for subsequent services).

2. Wipe the surface of the cell culture bottle with 75% alcohol and observe the cell status under a microscope. Due to transportation issues, a small amount of adherent cells may detach from the bottle wall; Do not open the culture bottle cap yet, place the cells in the cell culture box and let them stand for 2-4 hours to stabilize the cell state.

3. Carefully read the cell manual to understand cell related information, such as adhesion characteristics (adhesion/suspension), cell morphology, basic culture medium used, serum ratio, required cytokines, passage ratio, fluid exchange frequency, etc.

4. After settling, take out the cell culture bottle, examine it under a microscope, take photos, and record the cell status (the photos taken will be used as the basis for subsequent services); It is recommended to regularly take photos and record the growth status of cells after subculture.

5. Adherent cells: If the cell growth density exceeds 80%, it can be passaged normally; If the cell density does not exceed 80%, remove the culture medium from the cell culture bottle and reserve about 5ml for further cultivation until the cell density reaches around 80% before proceeding with the passage operation. The bottle cap can be slightly loosened.

6. Suspended cells: Transfer all the liquid in the cell culture bottle to a 50ml sterile centrifuge tube and centrifuge at 1200rpm for 5 minutes. After centrifugation, collect the supernatant culture medium for later use. Add 5ml of culture medium to the bottom of the tube and resuspend the cell precipitate. During microscopic examination, if the cell density exceeds 80%, the cell suspension can be cultured in two cell culture bottles and supplemented with culture medium up to 5ml. If the cell density does not exceed 80%, the cell suspension can be transferred to the original bottle for further cultivation until the cell density reaches around 80% before passaging.

HE agar plate (9cm)10 piecesSelective isolation and cultivation of Salmonella

Hektoen Enteric Agar

XLD medium plate (9cm)10 piecesSelective culture medium, mainly used for isolating Shigella genus, can also be used for isolating Salmonella

Xylose Lysine Desoxycholate Medium

Bismuth sulfite agar plate (9cm)10 piecesSelective isolation for Salmonella

Bismuth Sulfite Agar

Chinese Blue Agar Plate (9cm)10 piecesWeak selective culture medium for selective isolation of intestinal pathogenic bacteria

China Blue Agar

MacConkey agar plate (9cm)10 piecesSelective isolation and cultivation of intestinal pathogenic bacteria

MacConkey Agar

MacConkey Agar (SMAC) Plate (9cm)10 piecesIsolation and cultivation of Escherichia coli O157

Sorbitol Maconkey Agar Base

Crystal Violet Neutral Red Bile Salt Agar Plate (9cm)10 piecesSolid plate detection for coliform bacteria

Violet Red Bile Agar

Pinhong Ya * Agar Plate (9cm)10 piecesSelective isolation and confirmation of total coliforms in drinking water and source water

Fuchsin Basic Sodium Sulfide Agar

MFC agar plate (9cm)10 piecesFilter membrane method for detecting coliform bacteria

MFC Agar

Baird Parker agar plate (9cm)10 piecesUsed for selective separation and cultivation

Baird-Parker Agar Base

Bengal Red Medium Plate (9cm)10 piecesUsed for determining the total number of mold and yeast in food

Rose Bengal Medium

Oxford Agar (OXA) Plate (9cm)10 piecesSelective isolation of Listeria monocytogenes

Oxford Agar Base

PALCAM agar plate (9cm)10 piecesUsed for selective isolation of Listeria monocytogenes

PALCAM Agar

MRS agar plate (9cm)10 piecesUsed for determining the total number of lactic acid bacteria in food

MRS Agar

TCBS agar plate (9cm)10 piecesSelective isolation of pathogenic Vibrio species

Thiosulfate Citrate Bile Salts Sucrose Agar

Rose Red Sodium Agar Plate (9cm)10 piecesUsed for counting mold and yeast

Rose Bengal Medium

Agar plate (9cm)10 piecesIsolation and cultivation of Vibrio cholerae

Gentamycin Agar

BCYE agar plate (9cm)10 piecesSelective isolation and cultivation of Legionella bacteria

BCYE Agar Base

GVPC agar plate (9cm)10 piecesSelective isolation and cultivation of Legionella bacteria

GVPC Agar Base