The price of a fully automatic amino acid analyzer belongs to liquid chromatography, and its common flow path faults are mainly "blockage" and "leakage", but more importantly, "blockage".
Maintenance of price for fully automatic amino acid analyzer
The price of a fully automatic amino acid analyzer belongs to liquid chromatography, and its common flow path faults are mainly "blockage" and "leakage", but more importantly, "blockage". To effectively prevent and control flow path blockages, it is necessary to remember the eight words' disease enters through the mouth, prevention is the priority '. Below, we will elaborate from three aspects.
1、 Keep the entrance closed and prevent diseases from entering through the mouth
There are three channels for impurities to enter the amino acid analyzer: from the injector with the sample, with the buffer solution, and with the injector washing solution. Based on these three situations, the key to taking corresponding measures is to firmly control the "three checkpoints".
One is the sample processing. Strictly follow the procedure for sample processing, especially for physiological body fluid samples, be careful and cautious. Add too much precipitant, centrifuge for a longer period of time rather than shorter. After centrifugation, use a 0.22 μ m ultrafiltration membrane for ultrafiltration before starting the machine to prevent resin contamination and column head blockage.
Secondly, the buffer solution is closed. This step is often overlooked because when preparing reagents, filter paper is often used for filtration. Although the filtered reagents contain foreign objects that cannot be detected by the naked eye, in reality, there are still many small particles and pollutants in the liquid. These small particles have strong adhesion, and if not removed, they will cause pollution and blockage of the pump filter, pipeline, column head, and colorimetric cell.
The filter in the column head of the fully automatic amino acid analyzer is made of metal sintered, which has a very small pore size (about 0.2 μ m). If there are particles larger than this diameter in the solution, it will cause blockage or partial blockage of the filter. Mild cases may cause an increase in pump pressure, while severe cases may cause blockage of the analysis column. For this purpose, the procedure for preparing the buffer should be as follows: first, filter the buffer through a 360 mesh nylon mesh (coarse filtration), and then use a fully glass solution filter with a 0.45 μ m microporous membrane for ultrafiltration. The buffer solution for preparing indene ketone should also be prepared according to the above procedure. The third is to close the washing solution of the sampler. During work, the detergent must be changed daily. To prevent dust, insects, or other impurities from falling in, make an organic glass mask and cover it on top of the automatic sampler (reference size: 50cm × 38cm × 19cm). Leave a small hole 20cm below the front of the mask to facilitate the connection between the sampling pump and the sampling needle.