Pipette/pipette gun

Water Quality Testing Special - Pipette/Pipette GunExclusive supporting equipment for Yuan Yi Test, purchased equipment can be given away for freePipette/pipette gun

Calibration method for pipette:

Suggest regularly reviewingPipetteThe performance must be checked (such as adjusting according to usage frequency every 3 months), and performance testing must also be conducted after laboratory maintenance or repair. However, users should establish a regular testing protocol for pipettes based on the required accuracy, frequency of use, number of people using the pipette, nature of the pipette, and the larger allowable error accepted by the user. （ISO8655-1）

Attention: The performance of the pipette is only guaranteed when used in conjunction with the nozzle produced by the manufacturer.

1. Performance testing

Performance testing should be conducted without airflow and at temperatures between 15-30 degrees CelsiusCelsiusWithin the range (constant at ± 0.5 degrees Celsius) and indoors with humidity greater than 50%. The pipette, pipette tip, and pure water for testing should be placed in the testing room for a sufficient amount of time (at least 2 hours) to reach equilibrium with indoor conditions. Distilled water or deionized water (Grade 3, ISO3696) is used for testing. Weigh using an analytical balance with a testing accuracy of 0.01mg. （ISO 8699-6）

1. Set the required liquid transfer volume V0 (recommended to use 100%, 50%, 10% volume of nominal range).

2. Before the test, adjust the state of the pipette, install the suction head, and use the test liquid (distilled water) for five times of liquid suction and drainage.

3. Carefully aspirate the liquid, keeping the pipette upright while suctioning, with the pipette tip only 2-3mm below the water surface, and then aspirate the test water. Remove the pipette vertically and place the pipette tip against the side wall of the container.

4. Transfer distilled water to a weighed beaker and lightly touch the inner wall of the container with the suction head at a 30-45 degree angle above the liquid surface. Slide the suction head along the inner wall of the weighing container by 8-10mm and remove the pipette.

5. Read the weight in milligrams.

6. Weigh with a scale of one hundred thousandth, repeat 3-10 times, and record the results each time.

7. Calculate the volume based on the density of water. (Vi=mi * Z, where Z is the conversion factor)

8. Calculate the average volume V=(∑ Vi)/n, where n=number of weighings

9. Inaccuracy A%=(V-V0)/V0 * 100%

10. Inaccuracy

Compare the calculated inaccuracy and inaccuracy with the corresponding larger error. If the value is within the allowable range, the pipette can continue to be used. Otherwise, its accuracy and precision need to be rechecked, and if necessary, recalibrated.

2. Recalibrate

1) Insert the calibration tool into the hole of the calibration adjustment lock (below the button) (Fig. 7).

2) Rotate counterclockwise to reduce liquid volume or clockwise to increase liquid volume.

3) Repeat the performance testing steps to check the calibration status until accurate results are obtained.