PD-10 desalination chromatography column Sephadex G-25 packing Cytiva

PD-10 desalination chromatography column Sephadex G-25 packing Cytiva

PD-10 desalination column filled with Sephadex G-25 filler

The PD-10 desalination column contains Sephadex G-25 chromatography packing, which is used for rapid buffer replacement, desalination, and removal of small amounts of contaminants (salts, dyes, radioactive labels) from the sample through gravity flow or centrifugal precipitation.

Flexible operating plan - using gravity flow scheme (1.0 to 2.5 mL sample) or centrifugal precipitation scheme (1.75 to 2.5 mL sample) for desalination and buffer replacement

Flexible flux - can desalinate one sample or multiple samples in parallel

Good recycling - up to 70% to 95% or more

High desalination capacity - removes over 98% of salt (gravity flow scheme); More than 90% (centrifugal precipitation scheme)

Minimal dilution - there is usually no dilution when used in the form of a centrifugal chromatography column; When using the gravity flow scheme, the dilution is 1.4 times

Compared to dialysis, it saves time - a low-cost and convenient alternative solution that can replace time-consuming and cumbersome dialysis procedures

What items are included in the packaging of PD-10 chromatography column products?

Each product package includes 30 chromatography columns, one buffer tray, one chromatography column bracket, and 4 adapters for centrifugal precipitation.

Universal design, suitable for centrifugal sedimentation or gravity flow schemes

The product description covers both options. When using the gravity flow scheme, the sample can be easily processed through one or more parallel chromatography columns without the need for a purification system. The centrifugation scheme can run in parallel in a standard centrifuge, and the dilution of the eluted sample is low.

Successfully used these pre installed desalination columns

The following publications demonstrate the effectiveness of PD-10 chromatography columns:

Desalination of membrane proteins after anion exchange chromatography

Desalination of essential reductase variants involved in fatty acid biosynthesis

Buffer displacement of acyl carrier proteins with affinity tags

Accessories for PD-10 desalination column

The LabMate buffer reservoir can use a gravity flow scheme to achieve one-step application of chromatography column washing and equilibration buffer. This buffer reservoir is not included in this product, but can be purchased separately.

Other Cytiva Sephadex G-25 chromatography columns

The Cytiva desalination solution includes various chromatography columns containing Sephadex G-25 chromatography packing. Multiple products are available to meet sample volume requirements ranging from 100 µ L to 15 mL, and can be adapted to manual methods or chromatography systems. Let Cytiva help you find the right desalination product.

PD-10 desalination chromatography column Sephadex G-25 packing Cytiva

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