OptiPrep™ Density gradient medium

NegotiableUpdate on 05/06

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Overview

OptiPrep Density gradient medium, also known as iodinated diol, is a low osmotic pressure, non-ionic iodinated density gradient medium. It is a sterile and endotoxin tested 60% (w/v) iodoethanol aqueous solution used for in vitro separation of biological particles. It can be used for the separation of cellular and subcellular organelle components.

Product Details

OptiPrep ™ Density gradient medium, item number: D1556-250ML, also known as iodediol, is a low osmotic pressure, non-ionic iodine density gradient medium. It is a sterile and endotoxin tested 60% (w/v) iodoethanol aqueous solution used for in vitro separation of biological particles. It can be used for the separation of cellular and subcellular organelle components.

application：

OptiPrep ™ Can be used to separate molecules, cells, and subcellular organelles, including DNA, cytokines, lysosomes, endoplasmic reticulum, peroxisomes, T lymphocytes, and natural killer cells. [1] It is also used in the following application areas:

Study the efficiency of single cell encapsulation. [1]

Gradient based separation of aging cancer cells. [2]

Separate non enveloped virus particles from bronchoalveolar lavage fluid. [3]

Separate lipoproteins and macromolecules

Purification of virus particles

Purification of neuronal cells

Features and advantages

OptiPrep ™ It has many biocompatibility characteristics, making it an ideal choice for use.

It is biologically inert and does not interfere with the determination of marker enzymes. [1]

It is non-ionic, non-toxic, and has low permeability.

It can form a spontaneous gradient, which is more stable and easier to prepare than sucrose. [2]

Other instructions

60% (w/v) iodixanol aqueous solution (sterile).

Legal Information

OptiPrep is a trademark of Serumwerk Bernburg AG

Which document contains information on the shelf life or expiration date of a specific product?

If a specific product has this information, its recommended retest date or expiration date can be found in the analysis certificate.

How can I obtain information or analysis certificates related to a specific batch?

You can find a specific batch COA file by entering the batch number above in the "Files" section.

Why choose product D1556, OptiPrep, instead of other density gradient products such as Percoll or Histopaque?

Percoll and Histoaque must be removed by washing before use. If Percoll and Histoaque are not removed, these density gradients may be toxic to mammalian cells. In contrast, OptiPrep has no potential for bioaccumulation and can be considered non-toxic to cells. Compared to polysaccharide products such as Histoaque or Ficoll, OptiPrep also has lower viscosity.

What is the appearance of product D1556 OptiPrep?

The solution may appear transparent to slightly yellow.

How to find prices and inventory status?

There are multiple ways to find pricing and availability for our products. After logging into our website, you will find the displayed prices and availability on the product details page. You can contact any of our customer sales and service offices to obtain a quote. American clients.

What is the transportation information provided by the Ministry of Transport for this product?

Transportation information can be found in Section 14 of Product (M) on SDS. To access the transportation information for this material, use the link on the product details page.

How should I store the solution of product D1556, OptiPrep? ™ Density gradient medium?

After diluting product D1556, use OptiPrep ™ Density gradient medium, after reaching the desired density, the solution can be stored at 4 ° C. Before use, it should be thoroughly mixed to ensure that there is no settling material.

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