Nucleic Acid Extraction Instrument

The magnetic bead method virus total nucleic acid extraction kit uses pre packaged reagents and is a product specially designed for nucleic acid extraction. It is suitable for extracting virus DNA and RNA from samples such as 200 µ L serum, saliva, plasma, milk, swabs, cell-free culture medium, and tissue homogenate supernatant. The reagent kit is based on superparamagnetic magnetic particle purification technology, which can greatly reduce the risk of cross contamination and improve the sensitivity and accuracy of detection. The instrument only takes 40 minutes to run. The obtained DNA/RNA can be directly used for quantitative PCR or RT-PCR experiments.

Storage conditions:
1. The magnetic bead method virus total nucleic acid extraction kit is transported at room temperature and stored at room temperature after receiving the product, with a shelf life of 18 months.
2. Before using proteinase K, add 3.5 mL of proteinase dissolution solution. Stored at -20~8 ° C. SM-071171M-D01

Extraction process:
1. Remove the sealing film: Place the pre loaded reagent plate flat on the table and gently tap a few times to allow the liquid droplets on the hole wall to drip back into the hole, carefully removing the sealing film.
2. Add 200 µ L of sample and 30 µ L of proteinase K to the 2nd/8th row of wells. Pre install reagent in the 1st/7th row, 500 µ L of magnetic bead storage solution in the 2nd/8th row, 500 µ L of lysis buffer in the 3rd/9th row, 500 µ L of washing solution in the 4th/10th row, 500 µ L of washing solution in the 5th/11th row, and 100 µ L of washing solution in the 6th/12th row 32/48 channel nucleic acid extractor program design, named AE-1 number, hole position name, waiting time, mixing time, magnetic suction frequency, mixing speed, total volume, temperature status, 1 2 Hit 0 3 0 2 700 close, 1 Move 0 1 1 1 2 500 close, 2 Bind 0 10 2 2 700 50 degrees, 4 W1 0 1 1 2 500 close, 5 W2 0 1 1 2 500 close, 6 W3 6 1 1 2 500 close, 7 6 EB 0 8 3 2 100 55 degrees, 1 Drop 0 2 2 500 close
4. Click "Open Door", the instrument door will automatically open, and place the 96 well plate into the instrument.
5. Insert the magnetic jacket into the instrument.
6. Click on "Program Run" and select program AE-1. The instrument starts running. After about 40 minutes, the instrument will end.
7. Click "Open Door", the instrument door will automatically open, and then remove the magnetic jacket and 96 hole plate. Transfer the 6/12 column nucleic acid elution solution to a 1.5mL centrifuge tube.