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Nitric oxide content determination kit signal series - readily available in stock
NegotiableUpdate on 05/06
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Overview
Nitric oxide (NO) is widely distributed in the nervous, circulatory, respiratory, digestive, urinary and reproductive systems of living organisms, especially in nerve tissues where it is more abundant. $r $n Nitric Oxide Content Determination Kit Signal Series - Ready Stock
Product Details
Nitric oxide（Nitric oxide，NO）Content determination reagent kit instruction manual
Micro method 100 pipe/96 sample
Before formal testing, it is necessary to take 2-3 Make predictions based on samples with significant expected differencesMeasurement significance:
NO（Nitric Oxide，NO）Widely distributed in the nervous, circulatory, respiratory, digestive, urinary and reproductive systems of living organisms, especiallyIt is relatively abundant in neural tissue. As an information substance between and within cells, it plays a role in signal transmission and is a new type of biological messenger molecule that plays an important role in physiological and pathological processes of the body.
Measurement principle:
NO Highly prone to oxidation in the body or aqueous solutionNOUnder acidic conditions,NO2Generate diazo compounds with diazonium salt sulfonamines,Further coupling with naphthyl vinyl diamine, the product is 550nmThere is a characteristic absorption peak at this point, and by measuring its absorbance value, it can be calculated NOContent.
Nitric oxide content determination kit signal series - readily available in stock
composition
product name |
DC6004-100T/96S |
Storage |
Extraction solution: liquid |
100ml |
4℃ |
Reagent 1: Liquid |
6ml |
4℃light-proof |
Reagent 2: Liquid |
6ml |
4℃light-proof |
Instruction manual |
a copy |
|
Reagent 2: Liquid 6ml×1 Bottle,4℃Store away from light.(use before 60℃Heating oscillation 15min)
The specific product parameters are subject to the parameters in the product manual received
Self provided instruments and supplies:
Balance, mortar or homogenizer, visible spectrophotometer/ELISA reader, trace quartz colorimetric dish/96 Orifice plate, distilled water.
sampleplaceReason:
1. Organization: According to organizational quality（g）Volume of extraction solution(ml)for 1：5~10The proportion of（Suggest weighing the agreement 0.1gOrganize, join 1mlextractPerform ice bath homogenization on the liquid.10000g，4℃centrifugation 15minTake the supernatant and place it on ice for testing.
2. Bacteria and fungi: according to the number of cells（104 Individual: volume of extraction solution（ml）for 500~1000：1 The proportion of（suggestion 500 Ten Thousand Cells Plusenter 1ml extract solution）Ice bath ultrasonic disruption of cells（power 300w, Ultrasound 3 Seconds, intervals 7 Second, total time 3min）And then 10000g， 4℃, centrifugation 15minTake the supernatant and place it on ice for testing.
3. Other liquid samples such as body fluids and culture media: direct measurement.
Measurement steps and operation table:
1Preheating of spectrophotometer or enzyme-linked immunosorbent assay (ELISA) reader 30min Above, adjust the wavelength to 550nm.
2、operation sheet
Blank tube |
Measurement tube |
|
sample（μl） |
100 |
|
extract solution（μl） |
100 |
|
Reagent 1（μl） |
50 |
50 |
Reagent 2（μl） |
50 |
50 |
Mix well and let it stand at room temperature 15minIn a trace quartz colorimetric dish/96 Orifice plate, measurementA550，ΔA=A determination-A blank | ||
NO Content calculation:
a.The calculation formula for measuring with a trace quartz colorimetric dish is as follows
The standard curve regression equation is:y=0.016x -0.0103，R2=0. 9986 1Organizational samples:
（1）Calculated based on sample quality
NO Content（μmol/g Fresh weight)=（ΔA +0.0103）÷0.016×V Anti General÷（V sample÷V Sample General×W）×10-3
= 0.125×（ΔA +0.0103）÷W
（2）Calculated based on sample protein concentration
NO Content（μmol/mg prot）=（ΔA +0.0103）÷0.016×V Anti General÷（V sample×Cpr）×10-3
= 0.125×（ΔA +0.0103）÷Cpr
2Cells:
NO Content（μmol/104 cell）=（ΔA +0.0103）÷0.016×V Anti General÷（V sample÷V Sample General×Number of cells×10-3
=0.125×（ΔA +0.0103）÷Number of cells (10000)）
3Other samples:
NO Content（μmol/L）=（ΔA +0.0103）÷0.016×V Anti General÷V sample
= 125×（ΔA +0.0103）
V Counter total: total volume of reaction,0.2ml；V Sample: volume of sample in the reaction,0.1ml；V Sample total: Add the volume of extraction solution,1ml；Wsamplequalityg；CprProtein concentration,mg/ml
b.use 96 The calculation formula for orifice plate measurement is as follows
The standard curve regression equation is:y=0.008x -0.0103，R2=0. 9986 1Organizational samples:
（1）Calculated by sample weight
NO Content（μmol/g Fresh weight)=（ΔA +0.0103）÷0.008×V Anti General÷（V sample÷V Sample General×W）×10-3
= 0.25×（ΔA +0.0103）÷W
（2）Calculated based on sample protein concentration
NO Content（μmol/mg prot）=（ΔA +0.0103）÷0.008×V Anti General÷（V sample×Cpr）×10-3
= 0.25×（ΔA +0.0103）÷ Cpr
2Cells:
NO Content（μmol/104 cell）=（ΔA +0.0103）÷0.008×V Anti General÷（V sample÷V Sample General×Number of cells×10-3
=0.25×（ΔA +0.0103）÷Number of cells (10000)）
3Other samples:
NO Content（μmol/L）=（ΔA +0.0103）÷0.008×V Anti General÷V sample=250×（ΔA +0.0103）
V Counter total: total volume of reaction,0.2ml；V Sample: volume of sample in the reaction,0.1ml；V Sample total: Add the volume of extraction solution,1ml；Wsamplequalityg；CprProtein concentration,mg/ml
Nitric oxide content determination kit signal series - readily available in stock
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