New photochemical derivatizer

PHRE-15Photochemical derivatizer（Upgraded version）Photochemical derivative unit

Applicable for: detection of aflatoxin in traditional Chinese medicine

Scope of application:

This photochemical derivatizer is widely used for HPLC detection of aflatoxin and sulfonamide drugs, and it can enhance the detection of aflatoxin B₁And G₁Fluorescence intensity of aflatoxin B₁And G₁The detection limit can reach 0.1 μ g/L; It can also enhance the fluorescence intensity of sulfonamide drugs: sulfamethoxazole, sulfapyridine, sulfamethoxazole, sulfamethoxazole, sulfamethoxazole, sulfoquinoxaline, with a detection limit of around 10 μ g/L.

The photochemical derivatizer is placed between the chromatographic column and the detector for continuous post column photochemical derivatization reactions to improve the sensitivity and/or selectivity of fluorescence, UV, electrochemical detection, and chemiluminescence detectors.

Equipment Composition

The photochemical column derivative includes the following components:

Lamp holder with switch

UV lamp, 254nm (U.V. lamp, 254nm)

Knitted reactor coils with special process design for reactor holder

PEEK connector

Installment and use

Connect one end of the polytetrafluoroethylene tube on the derivatizer to the outlet end of the chromatography column and the other end to the inlet end of the fluorescence detector, without any connection direction requirements.

Chromatographic conditions

Chromatograph;

Fluorescence detector;

C18 column: 250mm (length) × 4.6mm (diameter), packing particle size 5 μ m;

Mobile phase: 55% methanol;

Flow rate: 1mL/min;

Column temperature: 40 ℃;

Excitation wavelength: 360nm;

Emission wavelength: 450nm;

Note: If the chromatographic columns are different (length, manufacturer or model), the mobile phase can be fine tuned between 40% and 60%.

Experimental diagram

Chromatograms of Total Aflatoxin Content before and after Four Derivatives

The contents of various toxins are: B₁=10μg/L、B₂=3μg/L、G₁=10μg/L、G₂=3μg/L

characteristic
- No need to use chemicals (reducing detection costs while also avoiding operator exposure to toxic chemicals);
- Adopting imported derivative pipelines and ultraviolet lamps, with stable quality and long service life;
The minimum detection limits for aflatoxins B1, B2, G1, and G2 are all less than 0.5ppb;
- Comply with AOAC 2005.08, AOAC 2008.02, AOAC Aa 11-05, Taiwan, China standard (SZ 0981800370) and European Pharmacopoeia 2.8.18 standard analysis method;
- Complies with Chinese national standards and import and export inspection standards

Technical Parameter
Dimensions (L * W * H): 350 * 155 * 125mm
Power supply: 220V/50Hz

UV lamp lifespan: approximately 2000 hours
Power: 8W
Derivative tube material: PTFE (polytetrafluoroethylene), length 15m
Maximum withstand voltage: 500psi (35 kg/cm2)
Weight: Approximately 2kg

Derivative pipeline inner diameter: 0.0075 inches

Comparison between the application of photochemical derivatizers and the non application of photochemical derivatizers