Multi detection gel chromatography (GPC/SEC)

POSTNOVA Company'sMulti detection gel chromatography (GPC/SEC)The system is different from the traditional gel chromatography method that only uses concentration detector. It combines concentration detector (differential refractive index or UV detector, etc.) with laser light scattering detector and viscosity detector, forming the concept of multidimensional detection of gel chromatography.

With the introduction of POSTNOVA's top 21 angle multi angle laser light scattering detector, gel chromatography has become an absolute and accurate method for measuring molecular weight and its distribution at one go, and at the same time, the radius of rotation of sample molecules Rg can be obtained, which makes a leap in the reliability and information content of gel chromatography results.

By outputting and analyzing data from an online viscosity detector, the intrinsic viscosity (or reciprocal of molecular density) of the sample can be obtained; The hydrodynamic radius of the sample; The structural information determined by the coefficients α and K of the Mark Houwink equation; Molecular structure (curling and stretching of chains); Branching analysis.

The multi detector technique of gel chromatography can not only characterize the absolute molecular weight and its distribution of macromolecules; It can also determine the size (Rg and Rh) and their distribution of molecules; The structure, conformation, association, and composition of copolymers of macromolecules. POSTNOVA provides various configurations of GPC/SEC systems and detectors for important natural or synthetic polymers, nanoparticles, and protein applications to address users' needs for characterizing different molecular properties and obtaining desired information. Gel chromatography and field flow separation technology can be used in parallel. Meet the needs of various users.

POSTNOVA Company'sMulti detection gel chromatography (GPC/SEC)NovaSEC is a professional application software for gel chromatography data processing in various ways. The functions include: curves of molecular weight (MW), intrinsic viscosity (IV), and rotation radius (Rh) versus rinse volume, differential and integral distribution curves of molecular weight, and calculation of branching degree of branched polymers; Calculate the copolymerization ratio and copolymerization distribution of the copolymer based on the ratio of the differential and UV detector signals (RI/UVratio); Plot the Mark Houwink equation using the intrinsic viscosity of the sample components and the calculated molecular weight data to obtain the K value; Calculate dn/dc, UV absorption constant, and A2 coefficient based on the accurate concentration and injection volume of the sample. Multi peak analysis and calculation can also be performed.