Mouse nitric oxide synthase interacting protein (NOSIP) ELISA kit KPR-H22424

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Overview

Product Introduction: This kit is used to determine the content of mouse Jagged2 protein (JAG2) in human serum, plasma, and related liquids. Our company specializes in ELISA kits, PCR detection kits, scientific research antibodies, bacterial strains, ATCC cells, biochemical reagents, immunological kits, etc!!!

Product Details

Product Name: Mouse Nitric Oxide Synthase Interacting Protein (NOSIP) ELISA Kit
Product Code: KPR-H22424

Storage conditions and expiration date:

1Storage of reagent kit:;2-8℃。

2Validity period:6 month

This kit is for research use only.

Purpose of using the mouse nitric oxide synthase interacting protein (NOSIP) ELISA kit:

This kit is used to determine mouse Jagged2 protein (JAG2) in human serum, plasma, and related fluidsContent.

Experimental principle of mouse nitric oxide synthase interacting protein (NOSIP) ELISA kit:

This kit uses the double antibody sandwich method to determine the expression of mouse Jagged2 protein (JAG2) in specimenslevel Using purified mouse Jagged2 protein (JAG2)Antibody coated microplates are used to prepare solid-phase antibodies, and Jagged2 protein (JAG2) is sequentially added to the micropores coated with monoclonal antibodies，Again withHRPMarked Jagged2 protein (JAG2)Antibody binding, forming antibodies-antigen-Enzyme labeled antibody complex, washedAdd substrate after washingTMBColor rendering.TMBinHRPUnder the catalysis of enzymes, it is converted into blue color, and under the action of acid, it is converted into final colorThe yellow color. The depth and pattern of colorsplanJagged2 protein (JAG2) in the productPositive correlation. Using an enzyme-linked immunosorbent assay (ELISA) reader450nmMeasurement at wavelengthAbsorbance（ODValue), through the standard curvecalculate
Mouse Jagged2 protein (JAG2) in the sampleConcentration.

Composition of Mouse Nitric Oxide Synthase Interacting Protein (NOSIP) ELISA Kit:

1.	Concentrated washing solution	7.	Termination liquid
2.	Enzyme linked immunosorbent assay (ELISA) reagents	8.	reference standard
3.	Enzyme labeled coated plate	9.	Standard diluent
4.	Sample diluent	10.	Sealing film
5.	Chromogenic agentAliquid	11.	Instruction manual
6.	Chromogenic agentBliquid	12.	self-sealing bag

Summary of operating procedures for mouse nitric oxide synthase interacting protein (NOSIP) ELISA kit:
·Prepare reagents, samples, and standards
·Add prepared samples and standards, react at 37 ℃ for 30 minutesbell·Wash the plate 5 times, add enzyme labeled reagent, and react at 37 ℃ for 30 minutes
·Wash the board 5 times, add color developing solutions A and B, and color develop at 37 ℃ for 10 timesminute
·Add termination solution
·Read OD value within 15 minutes

·Calculate

Mouse nitric oxide synthase interacting protein (NOSIP) ELISA kitcalculation:

Using the concentration of the standard substance as the horizontal axis,ODThe value is on the vertical axis, and a standard curve is drawn on the coordinate paper based on the sampleODFind the corresponding concentration from the standard curve; Multiply by the dilution factor again; Or use the concentration of standard substances andODCalculate the standard valueThe linear regression equation of the quasi curve, which represents the sample'sODSubstitute the value into the equation, calculate the sample concentration, and then multiply it by the dilution factor,It is the actual concentration of the sample.

noteImportant matters:

1The reagent kit should be taken out of the refrigerated environment and equilibrated at room temperature15-30It can only be used after minutes. If the enzyme-linked immunosorbent assay (ELISA) package is not opened

After use, the Flat noodles shall be stored in a sealed bag.

2Concentrated washing solution may precipitate crystals, and can be diluted by heating in a water bath to aid dissolution. Washing does not affect the results.

3Each step of sample addition should use a sampler and its accuracy should be regularly checked to avoid experimental errors. One sample addition time

control5 Within minutes, if there are a large number of specimens, it is recommended to use a sampling gun to add samples.

4Please make a standard curve and a duplicate hole at the same time as each measurement. If the content of the substance to be tested in the specimen is too high (sample)ODvalueLarger than standard holesODPlease dilute the sample diluent to a certain multiple first（nMeasure again after doubling, calculateWhen calculating, please multiply by the total dilution factor (x n x 5).

5The sealing film is only for one-time use to avoid cross contamination.

6Please store the substrate away from light.

7Strictly follow the instructions for operation, and the judgment of the test results must be based on the reading of the enzyme-linked immunosorbent assay reader.

8All samples, detergents, and various waste materials should be treated as infectious agents.

9Components from different batch numbers of this reagent must not be mixed.