Mouse macrophage activation factor (MAF) ELISA kit KPR-H23503

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Overview

Product Introduction: This reagent kit is used to determine the content of small mouse androgen binding protein (ABP) in human serum, plasma, and related liquids. Our company specializes in ELISA kits, PCR detection kits, scientific research antibodies, bacterial strains, ATCC cells, biochemical reagents, immunological kits, etc!!!

Product Details

Product Name: Mouse Macrophage Activation Factor (MAF) ELISA Kit
Product Code: KPR-H23503

Storage conditions and expiration date:

1Storage of reagent kit:;2-8℃。

2Validity period:6 month

This kit is for research use only.

Purpose of using the Mouse Macrophage Activation Factor (MAF) ELISA Kit:

This reagent kit is used for the determination of small mouse male binding protein (ABP) in human serum, plasma, and related liquidsContent.

Experimental principle of mouse macrophage activation factor (MAF) ELISA kit:

This reagent kit uses the double antibody sandwich method to determine the levels of mouse male binding protein (ABP) in specimenslevel Using purified mouse male binding protein (ABP)Antibody coated microplates are used to prepare solid-phase antibodies, and male binding protein (ABP) is sequentially added to the micropores coated with monoclonal antibodies，Again withHRPLabeled male binding protein (ABP)Antibody binding, forming antibodies-antigen-Enzyme labeled antibody complex, washedAdd substrate after washingTMBColor rendering.TMBinHRPUnder the catalysis of enzymes, it is converted into blue color, and under the action of acid, it is converted into final colorThe yellow color. The depth and pattern of colorsplanMale binding protein (ABP) in the productPositive correlation. Using an enzyme-linked immunosorbent assay (ELISA) reader450nmMeasurement at wavelengthAbsorbance（ODValue), through the standard curvecalculate
Small mouse male binding protein (ABP) in the sampleConcentration.

Composition of Mouse Macrophage Activation Factor (MAF) ELISA Kit:

1.	Concentrated washing solution	7.	Stop Solution
2.	Enzyme-labeled reagent	8.	reference standard
3.	Enzyme labeled coated plate	9.	Standard diluent
4.	Sample diluent	10.	Sealing film
5.	Chromogenic agentAliquid	11.	Instruction manual
6.	Chromogenic agentBliquid	12.	self-sealing bag

Summary of operating procedures for mouse macrophage activation factor (MAF) ELISA kit:
·Prepare reagents, samples, and standards
·Add prepared samples and standards, react at 37 ℃ for 30 minutesbell·Wash the plate 5 times, add enzyme labeled reagent, and react at 37 ℃ for 30 minutes
·Wash the board 5 times, add color developing solutions A and B, and color develop at 37 ℃ for 10 timesminute
·Add termination solution
·Read OD value within 15 minutes

·Calculate

Mouse macrophage activation factor (MAF) ELISA kitcalculation:

Using the concentration of the standard substance as the horizontal axis,ODThe value is on the vertical axis, and a standard curve is drawn on the coordinate paper based on the sampleODFind the corresponding concentration from the standard curve; Multiply by the dilution factor again; Or use the concentration of standard substances andODCalculate the standard valueThe linear regression equation of the quasi curve, which represents the sample'sODSubstitute the value into the equation, calculate the sample concentration, and then multiply it by the dilution factor,It is the actual concentration of the sample.

noteImportant matters:

1The reagent kit should be taken out of the refrigerated environment and equilibrated at room temperature15-30It can only be used after minutes. If the enzyme-linked immunosorbent assay (ELISA) package is not opened

After use, the Flat noodles shall be stored in a sealed bag.

2Concentrated washing solution may precipitate crystals, and can be diluted by heating in a water bath to aid dissolution. Washing does not affect the results.

3Each step of sample addition should use a sampler and its accuracy should be regularly checked to avoid experimental errors. One sample addition time

control5 Within minutes, if there are a large number of specimens, it is recommended to use a sampling gun to add samples.

4Please make a standard curve and a duplicate hole at the same time as each measurement. If the content of the substance to be tested in the specimen is too high (sample)ODvalueLarger than standard holesODPlease dilute the sample diluent to a certain multiple first（nMeasure again after doubling, calculateWhen calculating, please multiply by the total dilution factor (x n x 5).

5The sealing film is only for one-time use to avoid cross contamination.

6Please store the substrate away from light.

7Strictly follow the instructions for operation, and the judgment of the test results must be based on the reading of the enzyme-linked immunosorbent assay reader.

8All samples, detergents, and various waste materials should be treated as infectious agents.

9Components from different batch numbers of this reagent must not be mixed.