Mouse IL-4 precoated ELISPOT kit enzyme-linked immunosorbent assay kit 96T

Dayou is an independent brand of Dake, providing a complete set of ELISPOT/ELISA experimental products, serum-free ELISPOT culture medium and DC culture medium; Pre packaged with ELISPOT assay kit and reading board service for humans and mice; Pre packaged ELISA kits for humans, mice, and rats; Human separation liquid/tube and rodent and pig separation liquid. Medical grade separation solution (with medical device license) and separation tube, with low endotoxin content (less than 0.06EU/ml) and good separation effect.
ELISA, ELISPOT training, and sample testing services. Launch the Super series of immune cell culture products for cultivating immune cells such as DCs and CIKs.

Da You ® Enzyme linked Immunospot Assay Kit (ELISPOT)

Mouse IL-4 precoated ELISPOT kit enzyme-linked immunosorbent assay kit 96T

The most advanced technology for detecting the cellular immune level of organisms in the world today

ELISPOT technology is a highly sensitive analytical technique that combines cell culture technology and ELISA technology to detect in situ secreted factors of cultured cells in vitroDa YouThe ELISPOT kit pre packaged in the series can be illustrated using the following diagram:

A. The specific monoclonal antibody package is placed at the bottom of the culture well, and excess blank sites are blocked by non-specific protein solution;
B. Adding a single cell suspension and stimulating culture, positive cells are activated to secrete cytokines, which are captured by specific antibodies nearby;
C. Remove the cells and leave an "image" of cytokines at the bottom of the plate;
D. Adding enzyme labeled detection antibodies, detecting the binding of antibodies with "imaging" - cytokines, forming a "antibody antigen antibody" sandwich structure;
E. Adding a chromogenic substrate, under the catalytic decomposition of enzymes, insoluble pigments are generated, which precipitate on the membrane and form spots;
F. Spot counting and result analysis.

Da You ® Mouse precoated ELISPOT kit

★ Domestic * Pre packaged bedding, detachable board, easy to use

★ Affordable price and higher flexibility

All raw materials are imported, with stable quality and strong comparability of results

★ Single cell level detection, strong specificity, sensitivity up to one in 100000, remote detection technology

★ Rich product line, providing "one-stop" services including customized services, technical training, etc

Dayou ® The Mouse precoated ELISPOT Kit series is prepared using high affinity and high valence antibody pairs through processes such as pre coating with PVDF plates, low-temperature freeze-drying, and vacuum sealed packaging. The pre coated antibodies on the finished PVDF board have uniform distribution and stable potency. The experimental detection time has been shortened from 3 days to 2 days, significantly reducing the number of sterile experimental steps, alleviating the labor intensity of the experimenter, and reducing the chance of contamination. Enable experimenters to easily and efficiently complete complex ELISPOT detection experiments.

1.Mouse IFN-γ precoated ELISPOT KitmouseIFN-γPre packagedELISPOTtest kit

[Scope of Application] This kit is only used for scientific research and is not intended for clinical diagnosis.

【 Storage conditions 】 4 ℃

【 Validity Period 】 12 months

[Specification] 48T/96T/5 × 96T

IFN - γ, also known as type II interferon. In 1965, Wheelock et al. first discovered IFN like antiviral substances in the supernatant of PHA stimulated leukocyte cultures. In 1973, Younger and Salvin discovered the presence of an IFN in the supernatant of lymphocyte culture, but its antigenicity was different from previously discovered IFNs, so it was named type II IFN. In 1980, it was uniformly named IFN - γ. It is mainly produced by activated T cells (including Th0, TH1 cells and almost all CD8+T cells) and NK cells. IFN - γ can induce the expression of MHC class II antigens in monocytes, macrophages, dendritic cells, skin fibroblasts, endothelial cells, astrocytes, and other cells, allowing them to participate in antigen presentation and specific immune recognition processes. At the same time, it can also upregulate the expression of ICAM-1 (CD54) in endothelial cells, promote the expression of Fc γ R in macrophages, and synergistically complete various biological functions such as antiviral, anti-tumor, and immune regulation.

2.Mouse IL-4 precoated ELISPOT KitmouseIL-4Pre packagedELISPOTtest kit

[Scope of Application] This kit is only used for scientific research and is not intended for clinical diagnosis.

【 Storage conditions 】 4 ℃

【 Validity Period 】 12 months

[Specification] 48T/96T/5 × 96T

In 1982, Howard discovered a factor in T cell culture supernatant that promotes B cell proliferation, initially named B cell growth factor-1 (BCGF-1). Some laboratories refer to it as B cell stimulating factor-1 (BSF-1) or T cell growth factor-2 (TCGF-2). In 1986, the gene was successfully cloned and named interleukin-4 (IL-4). The mouse IL-4 gene is approximately 6kb long, and the mature IL-4 molecule consists of 120 amino acid residues. The naked peptide has a molecular weight of 14kDa and three glycosylation sites. After glycosylation, the molecular weight of IL-4 is 30kDa. IL-4 can promote the proliferation of B cells stimulated by SAC or anti IgM, and enhance the levels of IgG1 and IgE; It is also a growth factor secreted by T cells themselves. In addition, IL-4 has immunomodulatory effects on mast cells, macrophages, and fibroblasts.