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Shanghai Chuangling Biotechnology Co., Ltd
2nd Floor, No. 100 Guokang Road, Yangpu District, Shanghai
Mitsubishi Anaerobic Airbag Cell Culture in JapanD-07(5%Analysis of Technical Parameters for Carbon Dioxide: A Convenient Solution for Anaerobic Cell Culture
1. Basic Specifications
·Product model:AnaeroPack D-07
·Applicable container: Sealed culture tank (single bag applicable volume)2.5L)
·Usage: Disposable, ready to use after opening
2. Gas concentration control parameters
·Oxygen(O₂) Final concentration: Reduce to within half an hour of sealed reaction<1%Establish strict anaerobic conditions.
·Carbon dioxide(CO₂) Final concentration: synchronously generated5%ofCO₂ (fluctuation range ±)0.3%)Maintain the culture mediumpHstable.
·Dynamic stability: After reaching the target concentration, it continues24The fluctuation amplitude of gas composition within the hourly cultivation cycle is less than0.3%.
3. Physical parameters of the reaction process
·Pressure difference: The pressure inside and outside the container remains constant throughout the entire reaction process, with no positive or negative pressure generated.
·Temperature rise: The reaction releases heat, which is buffered by the gas bag itself. The temperature rise inside the sealed container is ≤0.5℃。
·Operation window: After opening the aluminum foil outer bag, it is necessary to5Complete container sealing within minutes.
4. Adaptability of cultivation cycle
·Gas maintenance capability: single use can sustainably maintain anaerobic and5% COThe environment is not less than48Hour.
·Cell experiment matching: The duration of routine cell hypoxia experiments is about24Hours, gas parameters remain unchanged throughout the entire process.
5. Storage and usage conditions
·Storage: Store at room temperature, away from light, in a dry environment, no need for refrigeration.
·Validity period: Under unopened conditions12A month.
·Working environment: Placed in a conventional constant temperature incubator for use, no external air source is required.
Supply Information
Shanghai Chuangling Biotechnology Co., Ltd Mitsubishi Gas Chemical(MGC)Authorized distributor of anaerobic cultivation products, Mitsubishi Anaerobic Airbag Cell Culture in JapanD-07(5%Carbon dioxide is available in large quantities in stock, with genuine factory products guaranteed. We welcome inquiries and orders from research institutions and university laboratories.