Manufacturer of fully automatic amino acid analyzer

The development of technology from manufacturers of fully automatic amino acid analyzers has made amino acid detection less problematic. Overall, the detection of amino acid toxicity is mainly manifested in

The principle of amino acid analyzer from the manufacturer of fully automatic amino acid analyzer

The amino acid analyzer of the fully automatic amino acid analyzer manufacturer adopts the classic cation exchange chromatography separation and indene ketone column derivatization method to analyze the component content of protein hydrolysate and various free amino acids. The basic structure of the instrument is similar to that of ordinary HPLC, but the amino acid analyzer has been optimized in detail for amino acid analysis (such as nitrogen protection, inert tubing, online degassing, elution gradient and column temperature gradient control, etc.). The classic cation exchange chromatography separation and indene ketone post column derivatization method are used to analyze the component content of protein hydrolysate and various free amino acids. The basic structure of the instrument is similar to that of ordinary HPLC, but detailed optimizations have been made for amino acid analysis, such as nitrogen protection, inert tubing, online degassing, elution gradient, and column temperature gradient control

Commonly used amino acid analyzers, high-performance liquid chromatography (HPLC), capillary electrophoresis (CE), etc. are used for amino acid detection. However, the limitations of these methods also restrict their widespread application. For example, when using an amino acid analyzer, there is too much background interference; Due to the wide variety of amino acids, it is difficult to find a suitable derivative reagent for HPLC detection, and when the content of a certain amino acid is too low, it can cause serious inaccuracies in the results; The stability of capillary electrophoresis (CE) method is poor, and the experimental results are even more difficult to be recognized.

The development of technology has made amino acid detection less problematic. Overall, the detection of amino acids mainly manifests in:

1. High precision and sensitivity. Amino acid detection can be accurately quantified up to 0.1 μ m using isotopes as internal standards. And it is not affected by the high and low content of each component, accurately measuring each amino acid and reducing the influence between amino acids.

2. Good stability and repeatability. The detection minimizes the impact of detection instruments on experimental results to the greatest extent possible, and the standard operating procedures and scientific experimental design have also been recognized by a large number of researchers. According to statistics, there are currently over 500 scientific papers using amino acid detection methods. And the use of literature for testing can gain recognition from more scholars and experts for its more scientific experimental data.

3. The use of amino acid detection can detect the content of up to 45 types of amino acids in the human body, and the detection principle is based on the structure and molecular weight of each amino acid, effectively avoiding mutual interference between amino acids, making the experimental results more scientific and the detection range wider. Detecting amino acids is currently the most extensive experimental method for detecting amino acids, with more scientific data and more detailed and stable spectra.