MCF 7B cell specific culture medium

Transportation and storage:

The company's products are only used for scientific researchDepending on the weather conditions and transportation distance, the company and the customer will negotiate and choose one of the following methods.

1) 1mL cryopreserved cell suspension is put into 1.8ml cryopreserved tube and transported in foam insulation box filled with dry ice; After receiving the cells, please thaw and revive them for cultivation as soon as possible. If resuscitation cannot be performed immediately, the frozen cells can be stored at -80 ℃ for one month.

2) The T-25 culture bottle is filled with culture medium and transported at room temperature; After receiving the cells, please observe the growth status of the cells under a microscope. If the bottle laying rate exceeds 85%, immediately perform passaging operation. If there are many suspended cells, please place the culture bottle in the incubator overnight to help non dead suspended cells adhere to the wall again.

Specification parameters:

Carefully optimized by the technical team and tested for a long time, this product can maintainThe optimal growth state of MCF 7B cells. This product already contains various ingredients required for the growth of MCF 7B cells, and no additional ingredients are needed. It can be directly used for in vitro culture of MCF 7B cells. This product is for further scientific research use only and should not be used for diagnosis, treatment, clinical, household, or other purposes.

Product Description

product form: Liquid

product concentration:1×

Product Specifications:125mL×4

culture medium composition:DMEM＋10% FBS＋1% P/S

Bacterial detection: Negative

fungal detection: Negative

Mycoplasma testing: Negative

Cell Growth ExperimentGood cell growth and normal morphology

Endotoxin content(EU/mL):≤3

Storage conditions: 2-8 ℃, stored away from light

transport conditionsIce bag refrigerated transportation

Validity period: 3 months

How to handle received:

1. Firstly, observe whether the culture bottle is intact and whether there is any leakage or turbidity in the culture medium. If there are any, please take photos and contact technical support in a timely manner (the photos taken will be used as the basis for subsequent services).

2. Wipe the surface of the cell culture bottle with 75% alcohol and observe the cell status under a microscope. Due to transportation issues, a small amount of adherent cells may detach from the bottle wall; Do not open the culture bottle cap yet, place the cells in the cell culture box and let them stand for 2-4 hours to stabilize the cell state.

3. Carefully read the cell manual to understand cell related information, such as adhesion characteristics (adhesion/suspension), cell morphology, basic culture medium used, serum ratio, required cytokines, passage ratio, fluid exchange frequency, etc.

4. After settling, take out the cell culture bottle, examine it under a microscope, take photos, and record the cell status (the photos taken will be used as the basis for subsequent services); It is recommended to regularly take photos and record the growth status of cells after subculture.

5. Adherent cells: If the cell growth density exceeds 80%, it can be passaged normally; If the cell density does not exceed 80%, remove the culture medium from the cell culture bottle and reserve about 5ml for further cultivation until the cell density reaches around 80% before proceeding with the passage operation. The bottle cap can be slightly loosened.

6. Suspended cells: Transfer all the liquid in the cell culture bottle to a 50ml sterile centrifuge tube and centrifuge at 1200rpm for 5 minutes. After centrifugation, collect the supernatant culture medium for later use. Add 5ml of culture medium to the bottom of the tube and resuspend the cell precipitate. During microscopic examination, if the cell density exceeds 80%, the cell suspension can be cultured in two cell culture bottles and supplemented with culture medium up to 5ml. If the cell density does not exceed 80%, the cell suspension can be transferred to the original bottle for further cultivation until the cell density reaches around 80% before passaging.

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