Inverted fluorescence microscope for live cells

Guangzhou Keshite Instrument Company recently launched a new high-performance inverted fluorescence microscope KOSTER IMC-600TFL,KOSTER IMC 600TFL Inverted Fluorescence MicroscopeSpecially designed for fluorescence microscopy imaging and transmission bright field observation in scientific research fields This system adopts an infinite beam path design, an efficient fluorescence excitation light path, a large numerical aperture flat field achromatic fluorescence objective lens, and a large field of view eyepiece, ensuring clear and bright imaging of the optical system and a wide field of view. The body design that meets the requirements of ergonomics makes it more comfortable and easy for you to operate. It is equipped with observation methods such as bright field, phase contrast, and fluorescence, and has adapters such as conventional culture dishes and bottles. It is particularly suitable for microscopic and fluorescence observation in fields such as biology, medicine, tissue culture, cell culture in vitro, plankton, environmental protection, food inspection, etc.

KOSTER IMC 600TFL Inverted Fluorescence MicroscopeEquipped with a specially designed microscope operator, live cell culture device, and corresponding adapter interface, it is convenient to conduct microscopic operation experiments and observe live cells for a long time. The KOSTER Imager Suite system control software can integrate live cell temperature and humidity into the same software, CO²Concentration, O²Display of cell culture conditions such as concentration, control of photography function, and recording of long-term sequence images for convenient recording of various cell experimental data.

IMC-600TFL inverted fluorescence microscope

Long life and wide spectral fluorescence excitation light source

Complete set of high-performance fluorescent objective lenses

KMC 200H&KMC 600H high-speed scientific research grade cameras

KOSTER Image Suite 1.0 Professional Application Software

High precision integrated live cell culture device

Microscopic operating system and sample preparation equipment

Product Features:

1. A newly designed fluorescent device with an exclusive long-life metal chloride high-efficiency fluorescent light source, directly connected without calibration. The fluorescent bulb has a lifespan of over 1500 hours, which is more than 7 times longer than traditional high-pressure mercury lamp fluorescent light sources, making it more convenient to use; The wide spectral output range reaches 300nm-800nm, which is more suitable for exciting various conventional fluorescent dyes.

2.Six position objective disc, optional LED single wavelength fluorescence excitation light source, no fluorescence filter disc design, high-speed excitation wavelength switching, suitable for dynamic sample recordingVarious wavelength ranges of fluorescent filters are available, perfectly matching fluorescent dyes such as DAPI, BFP, eGFP, CY3, TexasRed, FITC, etc., to achieve the best fluorescence effect. The LED single wavelength excitation light adopts high-speed electronic switching, with a switching speed of up to 20us. The KOSTER Imager Suite software is used to integrate and control the LED light source wavelength switching, power adjustment, and time series photography functions, which are suitable for proportional fluorescence concentration experiments.

3.Complete set of high-performance fluorescent objective lenses

The fluorescence objective lens adopts a special design of low short wave absorption optical materials, greatly improving the transmittance of various excitation light (including UV). Combined with a new fluorescence device, it provides high brightness, high definition, and high contrast fluorescence microscopy images, and is equipped with a long working distance objective lens suitable for observing samples in culture dishes, culture bottles, and other devices.

4. Research grade high-speed fluorescence detection digital camera KMC 200H &KMC 600H

By using the KMC 200H scientific grade CMOS digital imaging system with 2.3 million pixels, 1/1.2 inch large area, 24 bit color digital performance, and high speed, ultra-high resolution and high depth microscopic color images can be obtained under conditions such as bright field, fluorescence, dark field, phase contrast, and polarization in microscopes. In low light conditions (illumination), KMC 200H (scientific grade) can provide ultra-high quality images under long-term exposure. KMC 200H digital imaging system with new imaging control for Windows system andKOSTER Image Suite 1.0 application software.

Through the KMC 600H scientific grade CMOS digital imaging system with 6.3 million pixels CCD, 1/1.8 inch large area, and 24 bit color digital performance, ultra-high resolution and high depth microscopic color images can be obtained under conditions such as bright field, fluorescence, dark field, phase contrast, and polarization of the microscope. In low light conditions (illumination), KMC 600H (scientific grade) can provide ultra-high quality images under long-term exposure. KMC 600H (Research Grade) Digital Imaging System with New Imaging Control for Windows SystemKOSTER Image Suite 1.0 application software.

KOSTER Image Suite 1.0 applicationsoftwareIt is a microscopic imaging software that matches KOSTER series microscopes and cameras. It adopts a modular design and includes functions such as image preview, acquisition, analysis, processing, sharing, time series shooting, multiple image overlay, morphological parameter measurement, etc., bringing users the latest image processing experience. The image software features include image acquisition, image processing, timed shooting, morphological parameter measurement, data export, etc. It also supports multiple image output formats such as TIFF, JPG, BMP, and is compatible with third-party image processing software such as Image J and FIJI, making it convenient for image data editing and organization.

5.The KOSTER IMC-600TFL inverted fluorescence microscope provides a specially designed interface for expanding microscopy operations and live cell culture devices. The system has good integration performance and fully meets the needs of biomedical research.

systemconfigurationtable

Technical Specifications
eyepiece	Large field of view WF10X (field of view size Φ 22mm)
	centering telescope
Infinite flat field achromatic objective lens	objective lens		PLL 10X0.25 working distance: 4.3 mm, cover glass thickness: 1.2mm
			PLL 20X0.40 working distance: 8.0 mm, cover glass thickness: 1.2mm
			PLL 40X0.60 working distance: 3.5 mm, cover glass thickness: 1.2mm
	Phase contrast objective lens		PLL 10X0.25 PHP2 working distance: 4.3 mm, cover glass thickness: 1.2mm
			PLL 20X0.40 PHP2 working distance: 8.0 mm, cover glass thickness: 1.2mm
			PLL 40X0.60 PHP2 working distance: 3.5 mm, cover glass thickness: 1.2 mm
Eyepiece tube	Tilt at 45 degrees, with an adjustment range of 53-75mm for pupillary distance
Falling fluorescent lighting system	Metal chloride light source 75W
	LED single wavelength light source
	Fluorescent filter group
	group	type			Excitation plate wavelength	Transmitting film wavelength
	UV+V	Ultraviolet (UV)			320nm~380nm	435nm
		Purple (V)			380nm~415nm	475nm
	Blue+Green (B+G)	Blue (B)			450nm~490nm	515nm
		Green (G)			495nm~555nm	595nm
focusing system	Coarse micro coaxial, with locking and limiting devices, micro grid value: 2 μ m
converter	Six hole converter
Carrier platform	Fixed stage size: 227mmX208mm
	The size of the glass circular loading platform is Ø 118mm
	Mechanical movement size, movement range: longitudinal 77mm, transverse 114mm,
	Culture dish tray one			Inner groove size: 86mm (width) X129.5mm (length), suitable for circular culture dishes with a diameter of 87.5mm
	Cultivation dish tray 2			Inner groove size: 34mm (width) X 77.5mm (length), suitable for circular culture dishes with a diameter of Ø 68.5mm
	Petri dish tray three			Inner groove size: 57mm (W) X82mm (L)
Transmissive lighting system	Rotary phase contrast spotlight with a working distance of 55mm,
	6V30W halogen lamp, adjustable brightness
	Frosted glass, blue and green color filters

Optional accessories

eyepiece	10X division wide-angle eyepiece (Φ 22mm), grid value 0.1mm/grid
objective lens	Infinite flat field achromatic PL 5X/0.12 W.D26.1
converter	Six hole (internal positioning with inward facing ball)
Sample adapter	The maximum diameter of the hole seat is 50.5mm, the center distance is 63mm, and it is suitable for observation in a culture dish with an outer diameter of 50mm
	The maximum diameter of the hole seat is 35.5mm, suitable for observation in a 35mm outer diameter culture dish
	The maximum size of the hole seat is 51.5X74.5mm, suitable for observation in T25 culture dishes
	The maximum size of the hole seat is 66.5X66.5mm, suitable for observation in a 66X66mm square culture dish
	The maximum size of the hole seat is 76.6X26.2mm, suitable for observing standard glass slide samples
CCD connector	0.5X
	1X
	0.5X with dividing ruler, grid value 0.1mm/grid
Digital camera connector	CANON(EF), NIKON(F)
camera	KOSTER KMC-200H/KMC-600H research grade high-speed camera
Living cell device	Temperature control range: room temperature -45 ℃, CO2 concentration control range: 0.1% -20%, O2 concentration control range: 0.5% -20%, humidity control: 20% -99%; Gas flow rate control 5-20L/H; KOSTER IMC-600TFL microscope specific adapter 160mm X 110mm;
Microscopic manipulation device	MM0-202D high-precision electric 3D manipulator arm, MM-92/MM-92B， Pneumatic/hydraulic microinjector; KOSTER IMC-600TFL adapter;

System Schematic Diagram

Schematic diagram of KOSTER microscope operating system adapter

KOSTER KMC Camera Size and Spectral Diagram

Schematic diagram of KOSTER live cell culture system structure