Interleukin-8 ELISA KIT

NegotiableUpdate on 06/10

Model

Nature of the Manufacturer: Producers

Product Category

Place of Origin

Online Consult

Overview

Interleukin-8 ELISA KIT is used for quantitative detection of IL-8 levels in serum, plasma, or cell culture supernatants. Mainly used for evaluating inflammation response, infection, and tumor related research.

Product Details

Interleukin-8 ELISA KIT

Interleukin-8 ELISA KITIt is a quantitative detection tool designed specifically for the scientific research field, for scientific research use only, and cannot be used for clinical diagnosis. It is an important chemotactic cytokine mainly secreted by monocytes, macrophages, and other cells, participating in processes such as inflammatory response, immune response, and tissue damage repair. The kit has complete components and is easy to operate, which can accurately detect the corresponding substance content in samples and provide reliable data support for inflammation and immune related scientific research.

Detection Principle

This reagent kit adopts the principle of double antibody sandwich ELISA, pre packaged with corresponding specific antibodies on a microplate, which can specifically bind to the target substance in the sample; After adding the corresponding secondary antibody labeled with HRP, a sandwich complex is formed. Unbound components are removed by washing, and the substrate solution develops color under HRP catalysis. The absorbance is positively correlated with the concentration of the target substance, and quantitative detection can be completed by fitting a standard curve.

Product Features

1. Strong specificity: By using corresponding paired specific antibodies, cross reactivity with other cytokines, chemokines, and similar substances can be avoided, ensuring the authenticity of the results; 2. High sensitivity: The detection sensitivity is ≤ 10pg/mL, which can capture changes in the expression of low concentration target substances; 3. Good repeatability: The intra plate and inter plate coefficients of variation are ≤ 10% and ≤ 15%, respectively, and the results are stable; 4. Easy to operate: compatible with mainstream enzyme-linked immunosorbent assay (ELISA) analyzers, with short overall time consumption, and can be tested immediately upon opening the box; 5. Complete components: including a complete set of core reagents, no need to purchase additional auxiliary consumables.

Applicable sample types

This kit is suitable for scientific research samples such as serum, plasma, cell culture supernatant, inflammatory tissue homogenate, cerebrospinal fluid, etc. It can accurately detect the content of target substances in the samples. (Please refer to the product manual for specific sample processing methods)

Storage and precautions

###Storage conditions

Unopened reagent kits should be stored in a sealed container at 2-8 ℃, avoiding freezing and direct sunlight, and have a shelf life of 6 months; After opening, the core reagent can be stored at 2-8 ℃ for 14 days. The substrate solution should be stored away from light and should not be used after expiration.

###Precautions

Before the experiment, the reagents need to be equilibrated to room temperature, and the concentrated washing solution should be diluted proportionally; 2. When adding samples, the nozzle needs to be replaced to avoid cross contamination; 3. Strictly control the incubation and color development time to ensure accurate detection results; 4. Waste reagents and consumables must be disposed of according to scientific research standards; 5. For scientific research purposes only and cannot be mixed with components from other manufacturers.

Order Information

If you need to obtain detailed product information, batch quality inspection reports, or quotation plans, please feel free to contact Shanghai Baili Biotechnology Co., Ltd. for technical support and business services.

The above information is for reference only. Please refer to the product manual for details.