Interleukin-6 (IL6) ELISA kit (species: fish)

Interleukin-6 (IL6) ELISA kit (species: fish)

Name of reagent kit	Interleukin-6 (IL6) ELISA Kit
specification	48T/96T
brand	RENJIEBIO
detection method	ELISA enzyme-linked immunosorbent assay
detection wavelength	450nm
sample	Serum, plasma, tissue, cell supernatant, etc
Sample volume	50~100ul
research field	Neurobiology, metabolism, immunology, etc
scope of application	Only for scientific research experiments
save	2~8 ℃, stored away from light

Interleukin-6 (IL6) ELISA kit (species: fish)The process is as follows：
(1) Add 100 μ l of the test sample to each well of the test sample, and set up 3 parallel wells for each type of sample; Set up two negative control wells and add 100 μ l of untreated cell lysate to each well; Set up another blank control well and add 100 μ l of pure cell lysate.
(2) Place the enzyme-linked immunosorbent assay (ELISA) plate at 4 ℃ and coat overnight.
(3) Plate washing: Absorb the reaction solution in the well, rinse it once with washing solution (after filling the plate well with washing solution, shake it off), then fill the plate well with washing solution, soak for 1-2 minutes, and shake intermittently. Shake off the liquid inside the hole and pat dry on absorbent paper. Repeat washing 3-4 times.
(4) Add 50 μ l of PBS to each negative control well, and add 50 μ l of 1:500 diluted rabbit anti human AIF antibody working solution to each sample well and blank well.
(5) Place the enzyme-linked immunosorbent assay (ELISA) plate in a wet box at 37 ℃ and incubate for 60 minutes.
(6) Wash the board, same as (4).
(7) Add 100 μ l of HRP labeled goat anti rabbit antibody working solution diluted 1:5000 to each well.
(8) Place the enzyme-linked immunosorbent assay (ELISA) plate in a wet box at 37 ℃ and incubate for 60 minutes.
(9) Wash the board, same as (4).
(10) Add 100 μ l of TMB chromogenic solution to each well, gently mix for 10 seconds, and let it react in the dark at 37 ℃ for 15-20 minutes.
(11) Add 100 μ l of 2mol/L H2SO4 to each well to terminate the reaction.
(12) Measure the absorbance values W1 and W2 at 450nm and 630nm respectively, and the final measured OD value is the difference between the two (W1-W2) to reduce light interference caused by scratches or fingerprints on the container.
(13) Data processing: After obtaining the OD values of the specimen (S) and negative control (N), calculate the S/N value. S/N ≥ 2.1 is the positive judgment criterion.

Product advantages:

1、 Efficient, sensitive, and specific antibodies;

2、 Stable repeatability and reliability;

3、 Solid phase carriers with good adsorption performance, low blank value, and high transparency at the bottom of the pores;

4、 Suitable for various specimen types such as serum, plasma, tissue homogenate, cell culture supernatant, urine, etc;

5、 * Quality assurance, maximizing experimental cost savings.

Standard curve calculation

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