INDUCE-seq® Cracking off target challenges and safeguarding gene editing safety

INDUCE-seq ® Cracking off target challenges and safeguarding gene editing safety

Gene editing technology is expected to profoundly transform the modern medical paradigm. Precision gene editing tools, represented by CRISPR-Cas9, enable researchers to achieve targeted and programmable DNA sequence modifications at the genomic level,Enable humanity to overcome difficult diseases such as genetic disorders and malignant tumors from the root. butGene editing technology always hides a core industry challenge of off target effectsOff target editing refers to unexpected DNA modifications that occur at unexpected gene loci.Systematic identification and quantitative evaluation of off target effects have become important quality control processes in the development of gene editing therapies.However, currently within the industrycommonly usedoff-targetThe detection methods commonly suffer from limitations such as low throughput, cumbersome experimental procedures, complex data analysis, and poor reproducibility of results, making it difficult to meet the preclinical and clinical research needs of high sensitivity, high specificity, and large-scale validationIt is an important bottleneck that restricts the clinical translation of gene editing technology.

A biotechnology company from the UKBroken StringBiosciencesSuccessfully developeda kind ofCell based, PCR amplification freetechnologyplatform, able toHigh sensitivity, genome-wide rangeCapture and locate internallyDNA double strand breakofnew methodINDUCE-seq®.thatplatformNot only can it accurately identify double strand breaks induced by gene editing tools at predetermined target sites, but it can also systematically capture off target break events triggered in non targeted regions of the genome.INDUCE-seq®platformCan directly capture and locate single inducible and endogenous DNA double strand breaks in situ in living cells with high resolution，Can provide precise, quantitative, and unbiased data on targeted and non targeted editing activity across the entire genome within a few days，Significantly improve the decision-making timeliness and scientific rigor in the early stages of research and development, and assist the R&D team in completing critical risk assessments before key preclinical milestones such as candidate selection, toxicology research initiation, and IND application preparation. INDUCE-seq ®platformIt can deeply analyze the dynamic characteristics of gene editing, the specificity of nuclease cleavage, and the preference for cellular DNA damage repair pathways, thereby empowering researchers to optimize editing strategies, accelerate preclinical development processes, and substantially reduce the scientific and regulatory risks of therapeutic product development.INDUCE-seq®A set of workThe process has been integratedExperimental operations, high-throughput sequencing, and comprehensive bioinformatics analysis have achieved a closed-loop process from sample processing to data interpretation.

INDUCE-seq ® Having the following core advantages:

• Provide reliable and actionable high-quality data to comprehensively support decision-making at all stages of drug development;

• Covering the entire process from target discovery, preclinical research to clinical development, with end-to-end applicability;

• Implement autonomous collection, analysis, and management of critical security data to ensure data sovereignty and compliance;

• <span '='' style='; padding: 0px; overflow-wrap: break-word'>Support synchronous evaluation of on target and off target effects within the same standardized workflow, improving detection efficiency and comparability of results.

INDUCE-seq®Unbiased preferenceThe integration of detection technology with an integrated bioinformatics analysis platform aims to systematically transform high-throughput sequencing data into clear, structured, and clinically or research-oriented interpretations, thereby supporting the following key application scenarios:

Off target effect evaluation

• Design, screening, and prioritization of gRNA

• Optimization of gene editing efficiency and formulation of experimental strategies

• Analysis and dynamic modeling of targeted editing mechanism

• Functional characterization of nucleases and novel gene editors

INDUCE-seq ® Cracking off target challenges and safeguarding gene editing safety

INDUCE-seqThe platform provides two flexible and customizable delivery modes to meet the diverse needs of different customer groups such as research institutions, clinical testing centers, and biopharmaceutical companies:

1. It is a standardized complete set of reagent kits, covering all core components required for sample pretreatment, targeted induction editing, DNA extraction, library construction, and high-throughput sequencing adaptation. All reagents undergo strict quality control and batch validation to ensure experimental reproducibility and data reliability;

2. It's a full process techniqueTechnical services are provided by an experienced technical team, including experimental design, sample quality assessmentOne stop support for experimental operations, sequencing data generation, and basic bioinformatics analysis, especially suitable for users who have not yet established relevant technology platforms or need to quickly conduct methodological validation.

Both delivery forms can be personalized according to the specific application scenarios of customers, such as gene therapy off target effect evaluation, new base editor specific screening, CRISPR system optimization, etc., and are accompanied by detailed operation manuals, technical parameter explanations, and application case references to assist users in efficiently and accurately completing genome-wide detection and quantitative analysis of induced editing events.

Broken String BiosciencesisEnglandCardiff University's spin off companiesCommitted to helping gene editing teams accelerate the development of safer and more effective gene drugs.itsSuccessfully developedofa kind ofA new high-sensitivity and genome-wide DNA double strand break detection method, INDUCE seq ®，Not only can it accurately identify double strand breaks induced by gene editing tools at predetermined target sites, but it can also systematically capture off target break events triggered in non targeted regions of the genome.At present,Broken StringBiosciencesWe have established strategic partnerships with multiple gene editing companies worldwide to provide early and comprehensive editing effect evaluation services, thereby helping to enhance the scientific and reliable evaluation of product safety and accelerating the transition from preclinical research to clinical application. The core mission of this technology is to enhance the predictability and controllability of the gene editing process, and promote the steady development of gene editing technology towards safe, effective, and regulated clinical treatment plans.

Beijing Ximeijie Technology Co., Ltd. isBroken StringBiosciences is a first-class agent in China, always adhering to a professional and rigorous attitude to provide customers with high-quality products and services