Hyclone, pancreatin

Hyclone, pancreatin

Product Introduction

1、 Product Features
Collection method: During the production of fetal bovine serum, whole blood is collected using sterile disposable plastic bags, separated after coagulation, and the serum is collected and frozen for storage. The initial collection of fetal bovine serum is a key factor in controlling the quality of final serum products. Production can only be allowed when the initial raw materials meet our specifications.
2、 Recommended melting methods

15-20 ℃ water bath generally uses tap water, but please change the tap water in the water bath several times to avoid the temperature dropping too low. Alternatively, it can be washed directly underwater.

During the water bath process, the serum level should be immersed in water. Shake slowly during the melting process to mix the serum evenly, but do not produce foam
3、 Common issues with serum
The quality of fetal bovine serum is determined by the timing of blood collection, and there are strict standards for serum collection. The products produced by different times of blood collection are also different. Below are some small differences in fetal bovine serum and its related products sorted by Ruihua Biology.
(1) Fetal bovine serum: Eight month old fetal bovine heart puncture for blood collection;
(2) Newborn bovine serum: Venous blood collection from newborn cattle 12-24 hours after birth; It can be further divided into: a. Advanced newborn bovine serum: serum naturally precipitated after blood collection and settling; b. Standard newborn bovine serum: c. serum separated by centrifugation; d. Normal newborn bovine serum: serum with melted or slightly melted blood;
(3) Calf serum: Serum was isolated from arterial blood of three month old calves;
4、 Serum storage method
Standard fetal bovine serum should be stored at -5 ° C to -20 ° C. Our company is a manufacturer of "premium fetal bovine serum". Gibco fetal bovine blood serum is available in large quantities, with low prices and guaranteed quality! Welcome to come and purchase!
Precautions
Key points for serum preservation
(1) Serum that requires long-term storage must be stored in a low-temperature refrigerator at -20 ℃ -70 ℃. The storage time in a refrigerator at 4 ℃ should not exceed one month Due to the volume increase of about 10% when serum freezes, it is necessary to reserve a certain volume space before freezing it into a low-temperature refrigerator, otherwise contamination or glass bottle freezing may occur
(2) Heat inactivation refers to heating thawed serum at 56 ℃ for 30 minutes During the heating process, it is necessary to shake it regularly and evenly The purpose of this heat treatment is to inactivate complement components in serum Unless necessary, it is generally not recommended to perform this heat treatment, as it can cause a significant increase in serum sediment and also affect the quality of serum Complement participates in reactions such as cytotoxicity, smooth muscle cell contraction, histamine release from mast cells and platelets, enhanced phagocytosis, and promotion of chemotaxis and activation of lymphocytes and macrophages
(3) The thawing of bottled serum requires a gradual thawing method: the serum in a low-temperature freezer at -20 ° C to -70 ° C is dissolved in a refrigerator at 4 ° C for 1 day Then move to room temperature and wait for all to dissolve before packaging During the dissolution process, it is necessary to shake gently and evenly (be careful not to cause bubbles) to ensure uniform temperature and composition, and reduce the occurrence of precipitation Do not thaw serum directly from -20 ℃ to 37 ℃, as this can cause protein aggregation and precipitation due to significant temperature changes

(4) The serum provided by general manufacturers is sterile and does not require further filtration for sterilization If suspended particles are found in the serum, the serum can be added to the culture medium and filtered together. Do not filter the serum directly
(5) Sediment flocs in serum: mainly caused by the denaturation of lipoproteins in serum and fibrin in thawed serum, these flocs do not affect the quality of serum itself It can be removed by centrifugation at 3000rpm for 5 minutes, or it can be left untreated Microscopic 'small black dots': The formation of precipitates in heat-treated serum will significantly increase Some precipitates appear as' small black dots' under a microscope, often mistaken for serum contamination In general, this small black dot does not affect cell growth, but if serum quality is suspected, it should be immediately stopped and replaced with another batch of serum
(6) Do not leave the serum at 37 ° C for too long, otherwise the serum will become turbid, and the active ingredients in the serum will be broken, affecting the quality of the serum. Storage/transportation: -5 ° C to -20 ° C
5Hyclone, pancreatinthe way to distinguish
A pale yellow transparent liquid formed by the coagulation of blood. If blood is drawn from a blood vessel and placed in a test tube without anticoagulant, the coagulation reaction is activated and the blood rapidly coagulates, forming a gel. The condensed block shrinks, and the pale yellow transparent liquid that precipitates around it is serum, which can also be obtained by centrifugation after coagulation. During the coagulation process, fibrinogen is converted into fibrin blocks, so there is no fibrinogen in the serum, which is a major difference from plasma. In the coagulation reaction, platelets release many substances, and various coagulation factors also undergo changes. These components remain in the serum and continue to change, such as prothrombin turning into thrombin, gradually decreasing or even disappearing with the storage time of the serum. These are also differences from plasma. But a large amount of substances that do not participate in coagulation reactions are basically the same as plasma. To avoid interference from anticoagulants, many chemical components in blood are analyzed using serum as a sample.

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