Human mitogen activated protein/MAP (MAPK) ELISA kit

Human mitogen activated protein/MAP (MAPK) ELISA kit

Product Overview:

Proteins activated by human mitogen/MAP(MAPK)Core used for cell proliferation and stress signal research, precise detectionMAPKActivity. This is a key molecule in cell signaling, involved in cell proliferation, differentiation, apoptosis, and stress response. Its abnormalities are related to tumors and inflammation, and it is suitable for cell lysate and tissue samples, providing support for the study of cell signaling mechanisms and drug screening.

Kit composition:

Pre coated microplate: 96 well or 48 well specification, coated with RCA capture antibodies.

RCA standard: Provides original standard samples for drawing standard curves.

Label detection antibody: a detection antibody that specifically recognizes RCA.

Streptomycin HRP conjugate: used for signal amplification.

Substrate solution (TMB): Chromogenic substrate, turns blue when exposed to HRP enzyme.

Termination solution: Terminate the reaction, and the color of the solution changes from blue to yellow.

Washing buffer (10 x concentrated solution): used for cleaning microplates.

Sample: Used for diluting samples and standards.

Sealing film: prevents cross contamination.

Product Features:

High sensitivity and specificity: The double antibody sandwich method is used to ensure the accuracy and reliability of the detection.

Easy to operate: Provides complete reagents such as pre coated microplates, standard samples, labeled detection antibodies, etc., with standardized experimental procedures.

Widely applicable: Suitable for various sample types such as serum, plasma, and tissue homogenates, supporting scientific and clinical research.

Sample requirements:

Sample type:Serum, plasma, tissue homogenate, cell culture supernatant, etc.

Sample processing:

Serum/plasma:After collection, let it settle and centrifuge to remove sediment. Pack and store at -80 ℃ to avoid repeated freezing and thawing.

Tissue homogenateAdd the lysate homogenate in proportion and centrifuge to obtain the supernatant.

Cell culture supernatant: Centrifuge to remove debris and detect.

Attention:Avoid hemolysis or high-fat samples, fresh samples are preferred, and long-term storage requires -80 ℃.

Operation steps:

1、 Prepare reagents:

Equilibrate the reagent kit to room temperature 20 minutes in advance, and dilute the concentrated washing solution with distilled water.

2、 Sample addition:

Gradient dilution of standard samples: Dilute the original standard sample proportionally.

Sample processing: Take 50 μ L directly or diluted and add it to the micropores.

3、 Nurturing:

Add samples/standards and incubate at 37 ℃ for 1 hour.

After washing 3 times, add labeled antibodies and incubate at 37 ℃ for 1 hour.

Wash again, add streptavidin HRP, and incubate at 37 ℃ for 30 minutes.

4、 Color rendering and termination:

Add TMB substrate and incubate in the dark for 10-30 minutes.

Add termination solution to stop the reaction.

5、 Testing:

Immediately measure the absorbance (OD value) using an enzyme-linked immunosorbent assay (ELISA) reader at a wavelength of 450nm.

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