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Human Acid Sphingomyelinase (ASM) ELISA Kit
NegotiableUpdate on 05/06
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Overview
The human acidic sphingomyelinase (ASM) ELISA kit is designed specifically for studying sphingolipid metabolism and detecting ASM activity with specificity. This enzyme is involved in the degradation of sphingolipids, and its functional abnormalities are associated with sphingolipid storage disorders such as Niemann Pick disease. It is suitable for tissue homogenates and serum samples, providing a core basis for the diagnosis of diseases related to sphingolipid metabolism disorders.
Product Details
Product Overview:
Human acidic sphingomyelinase(ASM)Specially designed for research on sphingolipid metabolism, with specific detectionASMActivity. This enzyme is involved in the degradation of sphingomyelin, and its abnormal function is related to Nieman-The diagnosis of diseases related to sphingolipid storage, such as Pick's disease, is based on the adaptation of tissue homogenates and serum samples, providing a core basis for the diagnosis of disorders related to sphingolipid metabolism.
Product Name |
English name |
ASM Elisa Kit |
|
Item Number |
Y-E3026 |
specification |
48T/96T |
save |
Store in the dark at 2-8 ℃ |
purpose |
For scientific research experiments only |
Main components:
1. Pre packaged antibody enzyme-linked immunosorbent assay plate: 12 wells x 8 strips (detachable), stored at 2-8 ℃.
2. Standard product: Freeze dried powder (known concentration of α - BGT), dissolved and stored separately to avoid repeated freeze-thaw cycles.
3. Antibody detection: HRP labeled anti - α - BGT antibody.
4. Sample: Used for sample preprocessing.
5. Concentrated washing solution: 20 x concentration, needs to be diluted with distilled water before use.
5. Chromogenic substrates: A solution (TMB) and B solution (H ₂ O ₂), stored away from light.
6. Termination solution: 2M H ₂ SO ₄, containing strong acid, should be handled with caution.
7. Auxiliary materials: sealing film, self sealing bag, instruction manual.
Main features:
High sensitivity: capable of detecting alpha BGT concentrations as low as 1.0 ng/mL.
Wide applicability: Suitable for various samples such as humans, mice, guinea pigs, rabbits, pigs, dogs, cows, sheep, chickens, ducks, monkeys, plants, etc.
Easy to operate: The experimental steps are simple and suitable for scientific research use.
Product specifications and storage
Specification: 96T/48T.
Storage conditions: Store in the dark at 2-8 ℃, with a shelf life of 12 months.
Attention: After removing the reagent kit from the refrigerated environment, it should be equilibrated at room temperature for 15-30 minutes before use; If the enzyme label coated plate is not used up after opening, the Flat noodles shall be stored in a sealed bag.
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Inspection method:
1、 Reagent preparation
1. Washing solution: Dilute the concentrated solution 20 times with distilled water (e.g. 5mL concentrated solution+95mL distilled water).
2. Gradient dilution of standard samples: Prepare 6 concentration gradients using the sample (see instructions in the kit for the range).
2、 Operation steps
1. Balance: The enzyme label plate was balanced at room temperature for 30 minutes, and stored at 4 ℃ without sealing with Flat noodles.
2. Sample addition: Add 50 μ L of standard to the standard well, 50 μ L of test sample to the sample well, and no blank well. When the sample concentration is too high, it needs to be diluted and retested.
3. Incubation: Add 100 μ L of HRP labeled detection antibody to each well and incubate at 37 ℃ in the dark for 60 minutes.
4. Washing: Discard the liquid in the well, fill each well with washing solution, let it stand for 1 minute, shake dry, repeat 5 times.
5. Color development: Add 50 μ L of substrate A and B solution to each well, and incubate at 37 ℃ in the dark for 15 minutes.
6. Termination: Add 50 μ L of termination solution to each well and measure the absorbance (OD value) at 450nm wavelength within 15 minutes.
