Gene Pulser Xcell electroporation device

Gene Pulser Xcell electroporation device

Core advantages and features of the product

Fully functional integrated design: Gene Pulser Xcell is a complete system that integrates an electroporation instrument host and a multifunctional pulse module. It can achieve various waveform outputs such as square waves and exponential attenuation waves without additional accessories, meeting the specific needs of different cell types and experimental applications.

Performance and Reproducibility: The system provides precise voltage, capacitance, and resistance control to ensure highly accurate pulse parameters for each experiment, stable and reliable experimental results, greatly improving the reproducibility and success rate of experimental data.

Wide applicability: Standard operating procedures (SOPs) have been pre optimized and stored for various common mammalian cells, bacteria, yeast, and even plant protoplasts. Users can also easily customize and store personalized parameters, suitable for cutting-edge applications such as CRISPR gene editing, stem cell research, protein production, etc.

User friendly operating experience: equipped with a large color touch screen, the interface is intuitive and clear. The guided workflow makes method setting, experiment execution, and result recording simple and fast, significantly reducing the learning threshold for new users and improving laboratory work efficiency.

Safety and Compliance: Designed to comply with strict international safety standards, with multiple safety protection mechanisms to ensure the safety of operators and samples. The instrument operates stably with low maintenance requirements, making it an ideal choice for long-term research and production.

Concise operation and usage guide

The design philosophy of the Gene Pulser Xcell system is "complex technology, simple operation". The standard workflow is as follows:

Step 1: Experimental Preparation

Select the appropriate electric cup (such as 1 mm, 2 mm, 4 mm gap) based on the target cell type.

Prepare a cell suspension containing the molecule to be transfected (such as plasmid DNA) and add it to a pre cooled electroporation cup.

Step 2: Program Selection and Settings

Turn on the instrument and select "Protocols" from the main interface.

You can directly call the built-in pre stored optimization program, or create a custom program based on literature and experience. The key parameters include:

Waveform selection: exponential decay wave (commonly used for bacteria, yeast, and mammalian cells) or square wave (suitable for certain mammalian cells and difficult to transfect cells).

Parameter settings: Voltage, capacitance, resistance (or time constant) are precisely set according to the selected waveform.

Name and save the set program for easy access in the future.

Step 3: Perform electroporation

Insert the electric rotary cup containing the sample mixture into the sample slot of the instrument.

Confirm the parameters on the touch screen and press the "Pulse" button. The instrument will emit a prompt sound and complete pulse discharge.

The screen will display the actual transmitted pulse parameters (such as time constants) for recording and analysis.

Step 4: Sample Processing

After the pulse ends, quickly transfer the cells from the electric transfer cup to the preheated culture medium.

Subsequently, cultivate under standard conditions and wait for further testing and analysis.

Step 5: Data Management

The instrument can store a large number of experimental protocols and results, making it easy to trace and repeat experiments.

Gene Pulser Xcell electroporation device

More information

The transmission parameters that can be modified include time constant, actual applied voltage, pulse interval, and pulse time, depending on the selected waveform (exponential wave or square wave)

Provide a series of related programs for manual operation, preset experimental plans, user experimental plans, and optimized experimental plans

Gene Pulser with 0.1 cm, 0.2 cm, and 0.4 cm ®/ Micropulser ™ Compatible with electroporation colorimetric dishes

Compatible with any electroporation buffer, including Bio Rad's Gene Pulser electroporation buffer

Research instruments: Thermo Fisher/Albemarle/Olympus/Bole/Nikon/Leica/iCare/Aucma/Pentax

Research product categories: PCR instrument/fluorescence meter/ultra micro spectrophotometer/carbon dioxide incubator/micro desktop centrifuge/small and medium-sized desktop centrifuge/real-time fluorescence quantitative PCR instrument/cell counter/electrophoresis tank kit/protein transfer system/electroporation instrument/pipette/mixer/enzyme-linked immunosorbent assay reader/microscope/refrigerator/drying oven/shaker, etc

Intellectual Property Infringement Complaint: Hangzhou Zhongdeng Medical Technology Co., Ltd. does not produce medical products and scientific research instruments. All products come from the manufacturers, agents, and distributors of the products. If there is any infringement, please contact us and we will handle it as soon as possible!