Fluorescence quantitative detection service

MiRNA fluorescence quantitative detection service

Product Introduction

Real Time PCR， Real time fluorescence quantitative PCR, also known as quantitative PCR or qPCR,It is a method of using fluorescent chemicals to measure each amplification in DNA amplification reactionspolymerase chain reactionThe method of determining the total amount of products after the PCR cycle. A method for quantitative analysis of specific DNA sequences in a test sample using internal or external reference methods

Due to its advantages of simple operation, fast and convenient, high sensitivity, good repeatability, and low contamination rate, quantitative PCR is widely used in various fields such as medical testing, drug efficacy assessment, gene expression research, transgenic research, gene detection, pathogen detection, animal and plant detection, food detection, etc

Experimental Principle

The so-called real-time fluorescence quantitative PCR technology refers to the method of adding fluorescent groups to the PCR reaction system, using fluorescence signal accumulation to monitor the entire PCR process in real time, and finally quantitatively analyzing unknown templates through standard curves

Overview of real-time fluorescence quantitative PCR technology types: DNA binding dye method, probe based chemical method, quenching dye primer method

Experimental application

DNA and RNA quantification; Gene expression validation; Allele identification; SNP typing; Pathogen and virus detection; Multiplex PCR

experimental results