Fish Technology Service Technology

Fish Technology Servicestechnology

Product Introduction

Fish Technology ServicesFluorescence in Situ Hybridization is a type of technologyMethod for drawing physical diagrams， Label DNA (or RNA) probes with special nucleotide molecules, hybridize the probes directly onto chromosomes or DNA fiber sections, and then use monoclonal antibodies conjugated with fluorescein molecules to specifically bind to the probe molecules for qualitative, localization, and relative quantitative analysis of DNA sequences on chromosomes or DNA fiber sections

Experimental Principle

If the target DNA on the detected chromosome or DNA fiber slice is homologous and complementary to the nucleic acid probe used, a hybrid of the target DNA and nucleic acid probe can be formed by denaturation annealing renaturation of the two. Labeling a certain nucleotide of a nucleic acid probe with a reporter molecule such as biotin can be used to perform qualitative, quantitative, or relative localization analysis of the test DNA under a microscope using a fluorescence detection system through the immune chemical reaction between the reporter molecule and a specific affinity element labeled with fluorescein.

Experimental Procedure

Fluorescence in situ hybridization experimental procedure Preparation of FISH samples → Preparation of probes → Probe labeling → Hybridization → Chromosome banding → Fluorescence microscopy detection → Result analysis

experimental results