- Phone
-
Address
Room 1519-10, No. 32 Tieling Road, Yangpu District, Shanghai
Product Categories
Shanghai Renjie Biotechnology Co., Ltd
Fish Keratin Sulfate (KS) ELISA Kit
NegotiableUpdate on 05/06
- Model
- Nature of the Manufacturer
- Producers
- Product Category
- Place of Origin
Overview
The Fish Keratin Sulfate (KS) ELISA Kit provides rapid detection results within one week, with a complete range of indicators. Shanghai Renjie Biotechnology is committed to the research and sales of high-quality scientific research reagents. The company adheres to the concept of quality and customer first, operates with integrity, offers affordable prices, and guarantees quality. We have become a reagent supplier for numerous research institutes, enterprises, and institutions. We welcome your cooperation.
Product Details
Fish Keratin Sulfate (KS) ELISA Kit
Experimental principle:
Coat the target antibody withIn a microplate, a solid phase carrier is made, and standard samples or specimens are added to the micropores respectively. The target is bound to the antibody attached to the solid phase carrier, and then the microbial target antibody is added. After washing the unbound antibody, it is addedHRPLabel and avidin, wash again and addTMBSubstrate coloration.TMBUnder the catalysis of peroxidase, it is converted into blue, and under the action of acid, it is converted into the final yellow. The depth of color is positively correlated with the target in the sample. Using an enzyme-linked immunosorbent assay (ELISA) reader450nmMeasure absorbance at wavelength（O.D.Calculate the sample concentration based on the value.
Kit composition:
Test kit operation steps:
Self provided experimental equipment required for the experiment:
1. ELISA reader（450nm）
2. High precision sampler and gun head:0.5-10uLThe2-20uLThe20-200uLThe200-1000uL
3. 37℃incubator
4. Distilled water or deionized water
Experimental result calculation:
Draw standard curve: inExcelIn the worksheet, use the standard concentration as the horizontal axis, corresponding toODDraw a linear regression curve of the standard sample with values as the vertical axis, and calculate the concentration values of each sample according to the curve equation.
Example diagram of curve fitting method,The following data and curves are for reference onlyexampleIt is not related to the test results of this kit.
Test kit performance: Please request detailed electronic instructions from customer service personnel!
Technical tip:
1. When mixing or redissolving protein solutions, try to avoid foaming as much as possible.
2. To avoid cross infection, it is necessary to replace the nozzle when configuring different concentration standards, loading samples, and adding different reagents. Additionally, please use different pipettes for different reagents.
3. Please use the sealing adhesive correctly during each incubation to ensure the accuracy of the results.
4. The mixed chromogenic substrate should be colorless before being loaded onto the plate. Please store it away from light; If the microplate changes from being searched to different depths of blue.
5. The order of terminating the liquid on the plate should be consistent with the order of the color developing substrate on the plate; After adding the termination solution, the color inside the hole changed from blue to yellow; If there is green inside the hole, it indicates that the liquid inside the hole is not mixed evenly; Please mix thoroughly.
Product expiration date:6month
Fish Keratin Sulfate (KS) ELISA Kit
Similar Product Recommend
