Feto SDS-PAGE Coomassie Brilliant Blue Protein Gel Rapid Staining Solution

Product Name:Feto SDS-PAGE Coomassie Brilliant Blue Protein Gel Rapid Staining Solution

Product specification: 500ml/bottle; 1000ml/bottle

Product Code: 18.001.05/18.001.10

Komas Brilliant Blue Protein Gel Rapid Staining Liquid Product Introduction:

This Coomassie Brilliant Blue Protein Gel Rapid Staining Solution is a protein staining solution that can be instantly stained at room temperature, only staining the protein without staining the gel, without the need for decolorization, and is non-toxic and odorless.

This product is suitable for all kinds of denatured and non denatured protein gel dyeing, which can save a lot of time and accelerate the experimental process. It is recommended among many protein dyeing products because of its convenient and fast operation, good sensitivity, and green environmental protection.

Dyeing is fast and does not require decolorization, especially for protein purification experiments. After a few minutes, the next step of the experiment can be determined based on the protein staining band results. The whole process is easy to operate and store. No special equipment is required. The decolorized gel can be stored in pure water for several weeks. The principle of gel dye solution is the same as that of traditional dye test, and the same steps can be used to cut gel and mass spectrometry.

Feto SDS-PAGE Coomassie Brilliant Blue Protein Gel Rapid Staining SolutionUsage:

1. Remove the PAGE gel after electrophoresis and place it in a plastic container. Add an appropriate amount of staining solution to cover the PAGE gel, usually about 20-25ml depending on the size of the container.

2. Shake on a shaker. Do not touch the dye solution with bare hands as it is acidic. The bands begin to appear 1-2 minutes later. The staining bands will deepen over time. The usual staining time can range from 30 minutes to overnight. The background color does not increase significantly with dyeing time. The dye solution can be reused three times. As the number of uses increases, the staining speed slightly decreases, but it does not affect sensitivity.

3. After dyeing, place the protein gel in water for storage and take out for photos. Due to the different isoelectric points of proteins, the instantaneous adhesion ability of dyes is different. It is recommended to dye for 2h or overnight, and then put the gel into water for better results. In addition, the gel can be stored in water to purify the background.

Komas Brilliant Blue Protein Gel Rapid Staining Solution Product Features:

Dyeing is fast and does not require decolorization, especially for protein purification experiments. After a few minutes, the next step of the experiment can be determined based on the protein staining band results. The whole process is easy to operate and store. No special equipment is required. The decolorized gel can be stored in pure water for several months.

If the thickness of the glue exceeds 1mm, please add more dye solution and extend the dyeing time.

Komas Brilliant Blue Protein Gel Rapid Staining Solution Scope of Application:

This product is suitable for laboratory made or pre made SDS-PAGE and Native gels, and can also be used for staining residual proteins on Western transfer PAGE gels.

Tip: Like traditional Coomassie Brilliant Blue dye, this dye binds positively charged groups on proteins, and quantitative comparisons between different proteins need to consider the number of positively charged amino acids. This also explains why many users have observed that some proteins are easily stained while others are lightly stained. Dear, this is related to the amount and isoelectric point of lysine and arginine contained in the protein.

Common problem answers for Coomassie Brilliant Blue Protein Gel Rapid Staining Solution

Regarding product performance:

Compared to traditional dyes, how is this dye easier to operate?

Answer: The traditional PAGE gel used is Coomassie Brilliant Blue G-250, which requires a mixture of solution A and solution B, and can only be used once. It also needs to be boiled, deionized water, and water for 4 hours to decolorize. If you want to obtain a background free PAGE gel, it also needs to be left overnight. And our protein fast staining solution does not need to be so troublesome, nor does it require decolorization, and it is non-toxic, short in time, and highly efficient.

Why is decolorization not necessary?

Answer: After running the glue, the protein adheres to the glue. When using traditional staining solution to stain protein, the entire gel is stained, so it is necessary to wash off the staining solution on the gel background. But our protein staining solution has strong specificity, only staining protein without staining gel, so there is no need for decolorization.

How sensitive is the dye solution?

Answer: The sensitivity reaches nanogram level, and a large amount of experimental data shows that this dye solution can color the protein on the gel within 2 minutes, with high sensitivity.

4. How is product safety?

Answer: Traditional dye solutions contain toxic and irritating methanol. Our product replaces methanol with non-toxic ethanol, which has no irritating odor and is safer to use.

What are the advantages of this dye solution when conducting mass spectrometry or protein purification experiments?

Answer: If you are doing mass spectrometry and need to pursue weak band dyeing, traditional dyeing has certain advantages, but the traditional dyeing operation is complex and unsafe to use. Our product is environmentally friendly and easy to operate. If you are conducting protein purification experiments and want to quickly see the purification results, then our dye solution is a suitable product.

Komas Brilliant Blue Protein Gel Rapid Staining Solution About Product Usage:

After dyeing, the background of the glue is heavy and will turn blue. What should I do? Can it be washed with decolorization solution?

Answer: The background turns blue, which is a normal phenomenon. Simply place the glue in clean water and purify the background. If using a decolorizing solution for washing, the protein dye will also be washed off. Our dye solution does not require decolorization. Just place it in clean water to purify the background. If you have high requirements for the background, you can change the water purification twice more.

What should I do if the weak band staining is shallow?

Answer: This fast dye solution usually takes 2 minutes to develop color, but if it is a weak band and the dyeing time is not enough, the color will be very light, and fading may occur when water is added too early. To dye weak bands, it is generally recommended to dye overnight, change the water the next day, and purify the background.

Is there a relationship between dyeing effect and adhesive thickness?

Answer: Generally speaking, the thinner the glue, the faster and better the dyeing process. If your glue is thick, it is recommended to extend the dyeing time. If the thickness of the glue exceeds 1mm, please add more dye solution and extend the dyeing time.

Is it normal for trace precipitation to appear in the dye solution? How should we respond?

Answer: The occurrence of trace precipitation is normal and does not affect the effectiveness of use. During production, in order to enhance the specificity of the dye solution, only the protein is dyed and not the gel. One component is intentionally saturated, but the saturated solution will slightly change with temperature, resulting in a change in solubility and possible trace precipitation. However, as the temperature rises, it will dissolve without affecting its use.

How many times can the dye be reused?

Answer: In principle, the dye solution can be reused repeatedly. However, if it is used more than 3 times, the amount of SDS remaining in the dye solution may increase, resulting in silver flocculent or trace precipitation. In this case, filtering once is sufficient and does not affect its use. However, for the best experimental results, we recommend using it three times repeatedly. It is recommended to extend the staining time during the second and third staining sessions.

6. How long can the staining time be?

Answer: It is recommended to dye for 24 hours. If it exceeds 24 hours, the background may turn blue. If the background is already too blue, you can shake the glue in clean water overnight to lighten the background.

Kaomasi Brilliant Blue Protein Gel Rapid Staining Solution Regarding Product Preservation:

1. Will the dye solution be affected by light if it is not stored away from light?

Answer: Our dye solution is Swiss original and not affected by light. It can be placed in a regular acid and alkali resistant bottle.

Can the dye be stored at 4 degrees Celsius? What would happen if it was saved?

Answer: It is not recommended to store it four times. After using the dye solution once, some of the SDS in the gel will remain in the dye solution. After repeated use, the amount of SDS in the gel will increase. In addition, with low winter weather, precipitation will occur, which is a normal situation and does not affect use. It should be stored at room temperature. Do not store at low temperatures or 4 degrees Celsius.