Fecal occult blood qualitative detection kit (Piramide Cave method)

Cerebro spinal fluid (CSF) is a colorless and transparent liquid that exists in the ventricles, subarachnoid space, and central spinal cord. It is produced by the choroid plexus in the ventricles and has similar properties to plasma and lymphatic fluid. Normal adult cerebrospinal fluid is approximately 100-150ml, weakly alkaline, and does not contain red blood cells; Normal cerebrospinal fluid has a certain chemical composition and pressure, which plays an important role in maintaining relative stability of intracranial pressure. When the central nervous system is damaged, cerebrospinal fluid detection becomes an important auxiliary diagnostic tool. Total Protein (TP) is composed of albumin and globulin. The determination of total protein content in biological fluids (serum, urine, cerebrospinal fluid) generally relies on two assumptions: 1. All protein molecules are composed of pure peptides, with a nitrogen content of 16% by mass; 2. There are hundreds of protein molecules in body fluids, and each molecule has very similar characteristics for measuring reactions. Currently, commonly used methods for detecting total proteins include biuret method, UV spectrophotometry, dye binding method, Kjeldahl nitrogen determination method, precipitation method, etc.

The detection principle of this kit is that under acidic conditions, eosin dissociates into an anionic form, and the dye gradually fades away, resulting in a decrease in the blank absorbance of the reagent; The amino acid, histone acid, lysine, and amino acid residues in protein peptides dissociate into - NH3+groups, which bind to eosin to form a red protein complex. The absorbance is proportional to the protein concentration, and compared with the same treated standard solution, the protein content in the sample can be measured, which can be used for the determination of total protein content in human or animal cerebrospinal fluid samples. This kit is only used in the field of scientific research and is not suitable for clinical diagnosis or other purposes.

Fecal occult blood qualitative detection kit (Piramide Cave method) Fecal occult blood qualitative detection kit (Piramide Cave method)