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Elirat E-OSEL-M0008 QuicKey PromouseE2(Estradiol)ELISAtest kit
Elabscience Mouse E2 Estradiol ELISA Kit - Ready Stock
Product Item Number:E-OSEL-M0008
Product Name:QuicKey PromouseE2(Estradiol)ELISAtest kit
Product Specifications48T/96T/96T*5
Product Introduction:
Reactivity: Rat
Detection range:3.13-200 pg/mL
Sensitivity:1.88 pg/mL
The specific product parameters are subject to the parameters in the product manual received
Series Introduction:
traditionalELISACompared to the reagent kit, the experimental time has been shortened by at least1-2At the same time, the sensitivity of the reagent kit has been improved, allowing customers to obtain more accurate experimental results. This series isElabscienceThe independently developed new technology aims to help customers conduct scientific research in a more efficient way.
Purpose:
The reagent kit is used for quantitative detection of mouse serum, plasma, saliva, and urine in vitroE2concentration
Product application:
Enzyme linked immunosorbent assay(ELISA
Detection principle:
This test kit adopts competitionELISAThe law. Using miceE2The antigen is coated on an enzyme-linked immunosorbent assay (ELISA) plate, and samples (or standards) and horseradish peroxidase are added during the experiment(HRP)Marking resistanceE2Monoclonal antibodies, in samples (or standards)E2Compared to the envelope antigenE2Competitive combinationHRPMarking resistanceE2The binding site on the monoclonal antibody forms a coated antigenE2-HRPMarking resistanceE2Monoclonal antibody complexes, free components are washed away. Add chromogenic substrate(TMB),TMBUnder the catalysis of horseradish peroxidase, it appears blue and turns yellow after adding termination solution. Using an enzyme-linked immunosorbent assay (ELISA) reader450 nmMeasurement at wavelengthODValue,E2Concentration andOD450The values are inversely proportional, and the sample can be calculated by plotting a standard curveE2The concentration.
Reaction type: CompetitiveELISA
Reaction time:1 hour 30 minute
Reactivity: Rat
Detection method: colorimetric method; ELISA;Competition Law
Detection range:3.13-200 pg/mL
Sensitivity:1.88 pg/mL
Sample volume:50 microliter
Sample types: serum, plasma, saliva, urine
Specificity: Can detect mice in the sampleE2And there is no significant cross reaction with other similar substances
Precision: Both intra board and inter board coefficients of variation<10%
rate of recovery:80%-120%
Storage conditions:2-8°C
Validity period:6month
Elabscience Mouse E2 Estradiol ELISA Kit - Ready Stock
Product ordering information:
E-OSEL-M0008 QuicKey PromouseE2(Estradiol)ELISAtest kit 48T/96T/96T*5
For other item numbers, please consult our store customer service