Edge UV visible spectrophotometer

The Shanghai Lengguang UV visible spectrophotometer measures the absorbance of the component to be tested at a specific wavelength, compares it with a known concentration standard, and calculates the concentration of the component in the sample.

Shanghai Lingguang's UV visible spectrophotometer, as a universal analytical instrument, plays an important role in many fields:

1. Quantitative analysis - this is its main function

By measuring the absorbance of the component to be tested at a specific wavelength and comparing it with a standard substance of known concentration, the concentration of the component in the sample can be calculated.

In the field of life sciences:

Protein concentration determination: Using classical Bradford or Lowry methods, measure absorbance at specific wavelengths to calculate protein content.

Nucleic acid (DNA/RNA) concentration and purity detection: Measure the concentration at 260nm and determine the purity by the absorbance ratio of 260nm/280nm (e.g. the ratio of pure DNA is about 1.8).

Bacterial cell density measurement: Measure the absorbance (OD600) of the bacterial solution at around 600nm to monitor microbial growth.

In the field of chemistry and chemical engineering:

Determination of specific ion or compound content in solution: for example, detection of nitrate and phosphate in water, analysis of metal ion content, etc.

In the field of food and environment:

Food safety testing: such as the detection of additives (preservatives, pigments), heavy metals, and pesticide residues in food.

Environmental monitoring: such as the detection of chemical oxygen demand (COD), ammonia nitrogen, total phosphorus, heavy metals and other indicators in water quality.

2. Qualitative analysis

By scanning the absorption spectrum of the sample within a certain wavelength range (such as 190nm-1100nm) and comparing it with a standard spectral library, it can assist in determining the type or structure of the substance.

Material identification: For example, identifying different colored organic compounds because their chromophores are different, and the positions and shapes of their absorption peaks are also different.

Purity check: Pure substances have specific absorption spectra, and if impurities are present, the spectrum may exhibit abnormalities or additional absorption peaks.

3. Other applications

Dynamics research: Monitoring the concentration of reactants or products over time during chemical reactions to study reaction rates and mechanisms.

Determination of acid-base dissociation constant (pKa): The pKa is calculated by measuring the absorbance changes of a substance at different pH values.

Characteristics of Shanghai Lengguang UV Visible spectrophotometer:

1. The overall machine base and components are made of aluminum alloy die-casting, ensuring the quality and reliability of the instrument.

2. High quality C-T grating monochromator, precision grating and non spherical mirror optical components, excellent optical performance.

3. Adopting combined light source technology, using deuterium lamps with a working life of not less than 500 hours and Japanese Hamamatsu ultraviolet receivers, greatly improving the stability and reliability of the instrument.

4. Pre calibrated light source and spectral energy correction system, convenient for users to replace and maintain the instrument.

5. It can be connected to a serial printer through an RS232 serial communication interface, or optionally equipped with a dedicated data processing software package. After connecting to a PC, it can achieve functions such as data storage, recording, transmission, and linear regression

Technical parameters of Lengguang UV visible spectrophotometer:

Optical system: diffraction grating C-T monochromator

Light source: deuterium lamp (500 hour lifespan)+tungsten lamp

Wavelength range: 190~1100nm

Bandwidth: 2nm ± 0.4nm

Wavelength accuracy: ± 2nm

Wavelength repeatability: ≤ 0.5nm

Transmittance accuracy: ± 0.5% T

Transmission density repeatability: ≤ 0.2% T

Stray light: ≤ 0.2% T

Noise: ≤ 0.2% T (0% line), ≤ 0.5% T (100% line)

Range: 0.0~99.9% (T) -0.3~2.999 (A) 1~9999 (F) 0~9999 (C)

Display mode: 4-digit LED display screen

Interface type: RS232 serial port

Instrument size: 370mm × 320mm × 240mm

Weight: Net weight 7.5Kg

Standard configuration:

One UV visible spectrophotometer

One user manual

One copy of product qualification certificate

Four 1cm glass colorimetric dishes

Two 1cm quartz colorimetric dishes

One set of 1cm diameter colorimetric dish holder

One power cable

Two fuses (2A)

Packing list copy

Optional accessories:

Glass colorimetric dishes 10mm, 20mm, 30mm, 50mm

Quartz cuvettes 10mm, 20mm, 30mm, 50mm

10mm、50mm 光径比色皿架

Holmium oxide filter for accurate wavelength in the ultraviolet region

Praseodymium neodymium filter for accurate visible wavelength

Universal Data Processing Software Package (Complete Set)

Micro printer

Specialized communication cable

Specialized printing cable