Determination of Chloroestradiol in Feed by GE-200 Liquid Chromatography

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Nature of the Manufacturer: Producers

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Overview

GE-200 liquid chromatography is used to determine the content of estrone chloride in feed, and high-performance liquid chromatography is employed

Product Details

GE-200Determination of Chlorphenestrin in Feed by Liquid Chromatography

Referring to Announcement No. 1486-2010 of the Ministry of Agriculture (Determination of Chlorphenestrin in Feed by High Performance Liquid Chromatography), GE-200 liquid chromatography was selected for the determination of Chlorphenestrin content in feed, concentrated feed, and pre mixed feed additives, with a detection limit of 0.3mg/kg. Extract chlorpheniramine from feed using acetonitrile, centrifuge and collect the supernatantClassic C18 Solid Phase Extraction ColumnPurification, usingGE-200 liquid chromatography instrument-The wavelength of the UV detector is measured at 307nm. Calculate its content using the external standard method.

Materials and reagents

The water used for chromatographic analysis meets the requirements of Grade I water in GB/T 6682

Methanol: chromatographically pure

Acetonitrile: chromatographically pure

25% ammonia solution

Mobile phase: methanol+water=82+18

Chlorphenestrin stock solution: Accurately weigh an appropriate amount of Chlorphenestrin standard substance (purity ≥ 95%), dissolve it in methanol, and make up to 100ml. The concentration of the stock solution is 100ug/ml, sealed and stored in a refrigerator at 4 ℃, with a shelf life of one month.

Chlorphenestrin intermediate solution: Accurately weigh an appropriate amount of Chlorphenestrin storage solution and prepare it. Store it in a refrigerator at 4 ℃ with a shelf life of one week.

Standard working solution: Accurately transfer the reserve solution and intermediate solution of chlorpheniramine ether, and prepare them with methanol to form a standard working solution: 0.05 ug/mL、1.0ug/mL、2.0ug/mL、5.0ug/mL、10.0ug/mL、20.0ug/mL。 Ready to use.

Preparation of rinsing solution: 25% ammonia water+water=5+95

Classic C18 Solid Phase Extraction Column:200mg/3mL

It's a sample grinder used by CCTV

Analytical balance: sensitivity 0.1mg

Centrifuge: Speed above 5000r/min

Water Purification System

ultrasonic cleaner

Vortexers

oscillator

Sealed plastic centrifuge tube: 50ml

Solid phase extraction device

GE-200 liquid chromatograph: equipped with UV detector