D-Luciferin, sodium salt D-luciferin sodium salt

Product Name: D-Fluorescein Sodium Salt

English name:D-Luciferin,sodium salt
Product Specifications100mg/1g

CAS：103404-75-7
Molecular formula:C ₁₁ H ₇ N ₂ NaO3 S ₂ (anhydrous) or NaC ₁₁ H ₇ N ₂ O3 S ₂ · H ₂ O (monohydrate)
Molecular weight:302.3 g/mol (anhydrous) or 320.32 g/mol (monohydrate)
Appearance: A light yellow or pale yellow solid that is soluble in water.
Purity:≥ 99% (HPLC)
Storage conditions:-Dry at 20 ℃ and store away from light, with a shelf life of two years.

Product Introduction
1. Application areas of D-insect fluorescein sodium salt

Biological fluorescence imaging:

Real time observation of cell dynamics: In living cells or tissues,D-Sodium fluorescein can be used for real-time observation of molecular changes within cells, such as metabolic activity and signal transduction.

Tissue Engineering and Biotechnology: In tissue engineering cell culture,D-Sodium fluorescein can label specific cells or tissues for subsequent observation and analysis.

Fluorescent labeling technology:

Protein purification and research: as targets for biomolecules,D-Sodium fluorescein can bind with proteins to form covalent bonds, which are used for protein purification, structural studies, and interaction analysis.

Gene expression regulation research: By labeling specific genes or proteins, the mechanism of gene expression regulation and its role in diseases can be studied.

Biosensors:

Detecting molecular or environmental changes:D-Sodium fluorescein can be used as a component of sensors to detect specific molecules (such as glucose, hormones, etc.) or environmental changes (such as...)pHTemperature, etc., have potential medical applications.

Molecular Biology Research:

Research on protein-protein interactions: Used for labeling and studying protein-protein interaction networks, providing important tools for drug discovery and disease research.

Gene editing and repair technology: In gene editing and repair technology,D-Sodium fluorescein can be used as a marker to assist in target localization and efficacy evaluation.

2. Stability of D-insect fluorescein sodium salt

Stability is a key factor to consider when selecting reagents.D-Sodium fluorescein may decompose under light, but its stability is usually good, especially in anaerobic environments. Here are some explanations about its stability:

Light stability:

D-The sodium salt of insect fluorescein is easily decomposed under light, and the decomposition products may affect the experimental results. Therefore, experiments should be conducted in a dark environment or avoid prolonged exposure to natural light.

Acid alkali stability:

If under acidic or alkaline conditions,D-Sodium fluorescein may undergo chemical reactions, leading to decomposition or denaturation. Therefore, the solution should be controlled during usepHValue, avoid excessive acid-base conditions.

Thermal stability:

D-Sodium fluorescein may decompose or undergo polymerization reactions at higher temperatures, but it has better stability at room temperature. High temperature conditions, especially prolonged insulation steps, should be avoided during the experiment.

Photosensitivity:

Due to its fluorescent properties,D-Sodium salt of insect fluorescein is sensitive to light. In the experiment, direct exposure to light should be avoided to prevent interference from fluorescence signals.

3. Precautions

Reagent selection and preparation:

useD-When preparing insect fluorescein sodium salt, it is necessary to strictly follow the instructions or experimental plan to avoid excessive use, in order to prevent unnecessary toxicity or interference with experimental results.

Experimental condition control:

Maintain sterile conditions in the experimental environment, especially in biological fluorescence imaging and protein purification experiments, to avoid contamination by miscellaneous bacteria that may affect the experimental results.

Avoid high temperatures and strong light:

During the experiment, try to avoid direct contact with high temperature and strong light, especially in the fluorescence detection step, to prevent decomposition or signal interference.

Data analysis and result correction:

In fluorescence imaging experiments, it is necessary to calibrate the fluorescence signal to eliminate background noise and drift phenomena. At the same time, record detailed experimental data to ensure the reliability and accuracy of the results.
4. Experimental plan

Purpose of the experiment:

研究D-Application of insect fluorescein sodium salt in protein purification and fluorescence imaging.

Reagent preparation:

Prepare according to the instructionsD-The sodium salt solution of insect fluorescein is usually in dry powder form and needs to be dissolved in a solvent (such as anhydrous ethanol).

Protein sample preparation:

Mix protein samples (such as hemoglobin, albumin, etc.) withD-Mix the solution of insect fluorescein sodium salt and fully react to bind it with protein molecules.

Fluorescence confocal microscopy imaging:

Observe the sample using a fluorescence confocal microscope and record the fluorescence signals of cell surface proteins.

Analyze the distribution, aggregation status, and dynamic changes of proteins through microscopic images.

Fluorescence signal detection and analysis:

Use a fluorescence spectrometer to detect the intensity of fluorescence signals and analyze the fluorescence characteristics and interactions of proteins.

Compare different concentrationsD-The effect of insect fluorescein sodium salt solution on protein fluorescence signal.

Data recording and result analysis:

Record the experimental processD-Relevant data such as the concentration of fluorescein sodium salt, sample type, and fluorescence signal intensity.

Analyze data and exploreD-The mechanism of action of fluorescein sodium salt in protein purification and fluorescence imaging.

D-Sodium fluorescein is an excellent fluorescent labeling reagent widely used in fields such as biological fluorescence imaging and protein research. It has good stability, but attention should still be paid to controlling conditions such as light, acidity, and temperature in experiments. By strictly following the experimental plan and analyzing the experimental data reasonably, we can fully utilize itD-The role of fluorescein sodium salt in scientific research.