Ceramic Technique D-Fluorescein Potassium - Ready Stock

Pottery technique T4139 D-fluorescein potassium

Ceramic Technique D-Fluorescein Potassium - Ready Stock

Product Item Number:T4139

Product Name:D-fluorescein potassium

Product Specifications5mg/10mg/25mg/50mg/100mg/500mg/1g

Product Introduction:

D-Luciferin potassium (D-Luciferin K Salt) is a substrate of luciferase (Luc) that can catalyze bioluminescent insects to produce typical yellow green light (560 nm). D-Luciferin potassium can be used for luciferase assays.

The specific product parameters are subject to the parameters in the product manual received

Product Description: How to Translate

D-fluorescein potassium (D-fluorescein K salt) is a luciferase substrate that catalyzes the production of typical yellow green light (560 nm) in bioluminescent insects. D-fluorescein potassium can be used for luciferase assay.

In vitro activity:

Method: Used for in vitro bioluminescence analysis Preparation of D-Luciferin potassium:

1. Prepare 200 × D-Luciferin stock solution (30 mg/mL) with sterile water, gently mix until completely dissolved. After preparation, it can be used immediately or stored separately at -20 ℃.

2. Prepare 1 × D-Luciferin (150 µ g/mL) working solution in preheated tissue culture medium.

3. Suck out the culture medium for cultivating cells.

4. Add 1 × D-Luciferin solution to the cells before imaging. Cultivating cells at 37 ℃ for a short period of time before imaging can increase signal.

Internal activity:

Method: Used for in vivo bioluminescence analysis Preparation of D-Luciferin potassium:

1. Prepare fresh 15 mg/mL D-Luciferin stock solution using DPBS. Filter and disinfect with a 0.20 μ m filter.

2. Inject 10 µ L/g according to the weight of the mouse. Each mouse should be injected with 150 mg of D-Luciferin/kg body weight. For example, for mice weighing 10 g, injecting 100 µ L provides 1.5 mg of D-Luciferin.

3. Inject D-Luciferin intraperitoneally for 10-15 minutes during imaging. Fluorescein kinetics studies should be conducted for each animal model to determine peak signal time.

Kinase experiment:

In the absence or existence Under ENOblock or NaF conditions, pure enolase (3-9 U) was incubated at 37 ° C in a buffer containing 50 mM imidazole HCl (pH 6.8), and enolase activity was measured in 2.0 mM MgSO4 and 400 mM KCl. The reaction was initiated by adding 1 μ mol of 2-phospho-D-glyceryl ester, and OD was measured at 240 nm using a spectrophotometer after 10 minutes of reaction time.

Alias:D-Fluorescein Potassium Salt, D-Fluorescein Potassium Salt, D-Fluorescein Potassium Salt

Molecular weight:318.41

Molecular formula:C₁₁H₇KN series₂or₃S₂

CAS number: 115144-35-9

Storage: Avoid direct sunlight, store at low temperatures, store under nitrogen gas |Powder: -20 ° C for 3 years | Solvent: -80 ° C for 1 year | Transport using blue ice.

Solubility information

DMSO：2.63 mg/mL （8.26 mM）， Suggest ultrasound treatment.

H 2 O：50 mg/mL （157 mM）， Suggest performing ultrasound treatment.

Ceramic Technique D-Fluorescein Potassium - Ready Stock

Product ordering information:

T4139 D-Fluorescein Potassium 5mg/10mg/25mg/50mg/100mg/500mg/1g

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