Catecholamine (CA) ELISA Rabbit ELISA Kit

NegotiableUpdate on 05/06

Model

Nature of the Manufacturer: Producers

Product Category

Place of Origin

Online Consult

Overview

Catecholamine (CA) ELISA Rabbit ELISA Kit $r $n1. Accuracy: The correlation coefficient R value between the standard linear regression and the expected concentration is greater than or equal to 0.9900. $r $n2. Repeatability: The coefficients of variation within and between plates are both less than 15%. $r $n3. Storage: 2-8 ℃, keep away from light and moisture. $r $n4. Validity period: 6 months

Product Details

Catecholamine (CA) ELISA Rabbit ELISA Kit
Sample collection, processing, and preservation methods

1. Serum: Use test tubes free of pyrogens and endotoxins, avoid any cell irritation during the operation, collect blood, centrifuge at 3000 rpm for 10 minutes, and quickly and carefully separate serum and red blood cells.

2. Plasma: anticoagulant with EDTA, citrate or heparin. Centrifuge at 3000 rpm for 30 minutes and collect the supernatant.

3. Cell supernatant: Centrifuge at 3000 rpm for 10 minutes to remove particles and polymers.

4. Tissue homogenate: Crush the tissue by adding an appropriate amount of physiological saline. Centrifuge at 3000 rpm for 10 minutes and collect the supernatant.

5. Storage: If the sample is not tested in a timely manner after collection, please divide it into batches according to a single dose, freeze it at -20 ℃, avoid repeated freezing and thawing, thaw it at room temperature, and ensure that the sample is evenly filled and thawed.

Personal belongings

1. ELISA reader (450nm)

2. High precision sampler and nozzle: 0.5-10uL, 2-20uL, 20-200uL, 200-1000uL

3. 37 ℃ constant temperature box

Operation precautions

1. The reagent kit should be stored at 2-8 ℃ and equilibrated at room temperature for 20 minutes before use. The concentrated washing solution taken out of the refrigerator may crystallize, which is a normal phenomenon. Heating in a water bath causes crystallizationfullyDissolve before use.

2. The Flat noodles not used in the experiment shall be immediately put back into the self sealing bag and sealed (low-temperature dry) for storage.

The S0 standard with a concentration of 0 can be considered as a negative control or blank; When operating according to the instructions, the sample has already been diluted 5 times, and the actual concentration of the sample is obtained by multiplying the final result by 5.

4. Strictly follow the time, liquid dosage, and sequence indicated in the instructions for incubation operation.

Shake all liquid components thoroughly before use.

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