Capillary GC gas chromatography column for sterol aromatic compounds 25317

The Supelco SPB-5 gas chromatography capillary column with dimensions of 30 m × 0.53 mm X 0.50 μ m can be used for quantitative morphological analysis of nitrogen compounds using chemiluminescence detection. These compounds are polar and alkaline. It can be used as a stationary phase for the determination of deoxynivalenol and deoxynivalenol in grains by electron capture gas chromatography. It can also be used as a stationary phase for the determination of sterols in capillary column gas chromatography.

Capillary GC column, also known as open tube column. The carrier gas flows through the central hole and is not restricted throughout the entire column length.

Application: SPB-5 gas chromatography capillary column 25317 non-polar universal chromatography column mainly allows components to be eluted in order of boiling point, slightly improving selectivity, especially suitable for aromatic compounds.

USP code: The chromatographic column meets the requirements of USP G27 and G36.

Fixed phase: bound poly (5% diphenyl/95% dimethylsiloxane)

Temperature limit:

≤ 0.32mm inner diameter,<2 μ m: -60 ° C to 320 ° C (isothermal or programmed)

≤ 0.32mm inner diameter,<2 μ m: -60 ° C to 300 ° C (isothermal or programmed)

≥ 0.53mm inner diameter,<2 μ m: -60 ° C to 300 ° C (isothermal) or 320 ° C (programmatic)

≥ 0.53mm inner diameter,<2 μ m: -60 ° C to 260 ° C (isothermal) or 280 ° C (programmatic)

The column efficiency of Supelco SPB-5 gas capillary chromatography column in the United States is affected by internal and external factors. In order to achieve the best/-/efficiency of the chromatography column, in addition to reducing the dead volume outside the column, it is also necessary to have a reasonable column structure (minimizing the dead volume outside the packed bed as much as possible) and packing technology. Even with the best filling techniques, the filling situation at the center of the column and along the pipe wall is always different. The area near the pipe wall is relatively loose, which is prone to gully flow and faster flow velocity, affecting the manifold of the flushing agent and widening the spectral band. This is known as the pipe wall effect. This type of pipe wall area is approximately 30 times the thickness of the particle size from the pipe wall inward. In typical liquid chromatography systems, the effect of column external factors on column efficiency is much greater than that of tube wall effects.