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Shenzhen Dongfang Lantai Technology Co., Ltd

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    711, Building A, Jianjin Industrial Park, South of Donghuan 2nd Road, Longhua Street, Longhua New District, Shenzhen

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COSMOSIL 5C18-AR-II chromatographic column

NegotiableUpdate on 05/15
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Overview

The COSMOSIL 5C18-AR-II chromatographic column is a polymerized C18 reverse phase column. Due to the fact that its stationary phase octadecyl is bound to the surface of the packing silica gel through multi-point bonding, the stationary phase of this chromatographic column will not easily detach due to the presence of acid compared to a single point bonding chromatographic column. This chromatographic column is highly suitable for the analysis of acidic compounds and peptides under acidic mobile phase conditions.

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Product Description

COSMOSIL 5C18-AR-II chromatographic columnIt is a polymerized C18 reverse phase column. Due to the fact that its stationary phase octadecyl is bound to the surface of the packing silica gel through multi-point bonding, the stationary phase of this chromatographic column will not easily detach due to the presence of acid compared to a single point bonding chromatographic column. This chromatographic column is highly suitable for the analysis of acidic compounds and peptides under acidic mobile phase conditions.

  • Strong acid resistance

  • Suitable for acidic compounds and peptides


Acid resistance:

Comparison of decomposition test results of 0.1% trifluoroacetic acid aqueous solution at 60 ° C. The capacity factor (k ') is the naphthalene value when the mobile phase is methanol: water=70:30.

COSMOSIL 5C18-AR-II耐酸性

Application Case - Acidic Compounds

COSMOSIL 5C18-AR-II酸性化合物应用案例


Usage information

chromatographic column 5μm C18-AR-II
Inner diameter (mm) 1.0 2.0 3.0 4.6 6.0 10 20 28
Column length (mm) ALL ALL ALL ALL ALL 20-150 250 20-100 150-250 ALL
Factory solvent Acetonitrile/water=50/50 Acetonitrile/water=60/40 Methanol/water=70/30 Methanol/water=70/30 Methanol/water=80/20 Methanol/water=70/30 Methanol/water=80/20 Methanol/water=80/20
flushing method
  1. Method for removing buffer, salt, and acid from the chromatographic column: Rinse with a mobile phase free of buffer, salt, and acid for 10-15 minutes.
    Example: When the mobile phase is methanol/phosphate buffer (20mmol/L)=50/50, rinse with methanol/water=50/50

  2. The method of flushing the attached substances in the chromatographic column and the solution when the baseline is unstable

  • When the sample is not protein, rinse with methanol or *.

  • When the sample is protein, use 0.1%C2HF3O2Rinse with 50-70% acetonitrile/water





Shenzhen Dongfang Lantai Technology is a high-tech company specializing in the import of laboratory analytical instruments and equipment such as gas chromatography, liquid chromatography, electronic balance, electrochemical instruments, general instruments and equipment, chromatography columns, reagents, standards, etc. At the beginning of its establishment, the company insisted on focusing on the analysis industry and was committed to introducing domestic and foreign analytical instruments to a wide range of domestic users. Currently, the company's users have spread to scientific research institutions, colleges and universities, hospitals, chemical industry, food, medicine, agriculture, mining, power, metallurgy and other industries and units.