Biorad12003154ChemiDocMP gel Imaging System Product Introduction and Selection Guide Technical Guide

12003153 Product Introduction and Selection Guide

Product code: 12003153 Product name: Bio Rad ChemiDoc chemiluminescence gel imaging system

1、 Basic Product Information

12003153 is a multi-functional gel imaging system launched by Bio Rad, focusing on high-sensitivity chemiluminescence, fluorescence and visible light imaging, designed for Western blot, nucleic acid/protein gel quantitative analysis and other scientific research scenarios, integrating intelligent touch and automatic imaging technology, replacing the traditional dark room film method, to achieve efficient, accurate and repeatable digital imaging and data analysis.

core parameters

table

2、 Core functions and application scenarios

1. Core functions

·Chemiluminescence detection: comparable to X-ray film sensitivity, compatible with conventional chemiluminescence substrates such as ECL, without darkroom or film consumables.

·Fluorescence/colorimetric imaging: supports nucleic acid dyes (EB, SYBR series) and protein staining (Coomassie Brilliant Blue, Silver staining, SYPRO Ruby).

·Stain Free technology: No staining required, protein bands can be visualized directly after electrophoresis, supporting total protein normalization and quantification.

·Automated control: automatic focusing, automatic exposure, automatic recognition of sample trays, one click generation of publication level images.

·Data analysis: molecular weight determination, band quantification, signal-to-noise ratio calculation, data export Excel/GIF/JPEG.

·Compliance: Image Lab PC version supports FDA 21 CFR Part 11 and meets data audit tracking requirements.

2. Applicable applications

·Western blot chemiluminescence/fluorescence detection

·Nucleic acid agarose/polyacrylamide gel imaging

·Protein gel staining, silver staining and non staining imaging

·Spot hybridization, colony counting, and autoradiography

·Laboratory daily quantitative analysis and data archiving

3、 Product advantages

1. High sensitivity refrigeration CCD: -30 ℃ deep refrigeration reduces noise, weak signal bands are clearly detected, and quantification is accurate.

2. Integrated multifunctional: A single device covers the entire scene of chemiluminescence, fluorescence, colorimetry, and dye free, reducing equipment investment.

3. Intelligent touch operation: 9.7-inch touch screen, 3-step imaging, no need for professional training.

4. Non staining technology empowerment: Simplify the Western process, perform quality control throughout the entire process, and normalize total protein to improve data reliability.

5. Stable and reliable: Industrial grade optical system, with no drift during long-term use and easy maintenance.

6. Data compliance: Meet GLP/GMP laboratory data management and traceability requirements.

Biorad12003154ChemiDocMP gel Imaging System Product Introduction and Selection Guide Technical Guide

4、 Selection Guide

1. Applicable scenarios (recommended selection)

·Core requirements: Western blot chemiluminescence is the main requirement, and conventional gel/fluorescence imaging is also considered.

·Laboratory types: medium to large research laboratories, molecular biology/protein research platforms, pharmaceutical company R&D quality inspection.

·Budget and Performance: Adequate budget, pursuit of high sensitivity and multifunctionality, requiring long-term stable quantitative data.

·Space conditions: The laboratory countertop is spacious (≥ 65 × 55cm) and can accommodate medium-sized equipment.

2. Not applicable scenarios (cautious selection)

·Only basic nucleic acid gel imaging is performed (GelDoc Go 12009077 can be selected for limited budget).

·Requires multi-color fluorescence (≥ 3 channels) or near-infrared detection (recommended upgrade to ChemiDoc MP 12003154) Bio Rad.

·Ultra trace protein/extremely weak chemiluminescence signal (requires higher-level refrigeration CCD system).

3. Selection comparison (reference for the same series)

table

4. Suggestions for supporting selection

·Standard configuration: Blot/UV/Stand Free sample tray (covering mainstream applications).

·Optional: Blue light sample tray (safe nucleic acid imaging, alternative to UV).

·Software: Standard Image Lab Touch; The computer version of Image Lab needs to be selected in compliance.

·Maintenance: It is recommended to have a one-year original factory maintenance and regularly clean the optical system.

5、 Selection Summary

12003153 (ChemiDoc) is the mainstream choice for balancing performance, functionality, and cost, suitable for laboratories that focus on Western blot chemiluminescence and consider multi scene imaging.

· ✅ Choose it: requires high sensitivity, multifunctionality, automation, and compliant data.

· ❌ Not selected: only basic gel, very low budget, multi-color fluorescence/near-infrared

Biorad12003154ChemiDocMP gel Imaging System Product Introduction and Selection Guide Technical Guide

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