BD FACS Human Inflammation Cytokine Kit CBA Cytokine Assay

BD FACS Human Inflammation Cytokine Kit CBA Cytokine Detection Kit Flow cytometry

Becton Dickinson bioscienceBidi Life Sciences USABD Company CBA Cytokine Detection KitCytometric Bead Array（CBA）,Micro sample multi index flow cytometry protein quantification technology is a multi protein quantification detection method based on flow cytometry detection system, which can simultaneously detect multiple indicators in a single sample. In daily experiments, it is often necessary to quantitatively detect soluble proteins in the solution system, such as the quantitative analysis of cytokine content in cell culture supernatant or serum. Due to the low content of these factors, which is lower than the detection limit of general conventional methods, commonly used protein detection methods are usedwestern BlotIt's difficult to detect.BDThe company utilizes flow cytometry to amplify fluorescence signals in stages. By attaching soluble factors to particles with similar cell diameters, various soluble factors in the tested sample can be detected. To this end,BDThe company has developed a micro sample multi index flow cytometry protein quantification system, abbreviated asCBAThe system uses a series of small, dispersed microspheres with fluorescent labels to bind specific capture antibodies to capture the analyte in the solution system. By detecting the different fluorescence intensities on the specific microspheres corresponding to different analytes, the quantity of multiple soluble components in the analyzed sample can be simultaneously determined.BDofCBAThe working principle of the system is simple. Each detection indicator in the tested system is equipped with different capture microspheres. Different capture microspheres are coated with specific capture antibodies and have different fluorescence intensities. After mixing the capture microspheres with the test sample solution, the specific antibodies on the microspheres bind to the corresponding antigens or proteins in the sample (serum, plasma, or cell culture medium). Finally, fluorescent detection antibodies are added to form a "sandwich" sandwich complex. The content of each detection factor in the sample can be analyzed by flow cytometry fluorescence detection. Due toBDofCBAEach microsphere in the system can provide a surface that binds to a specific protein, therefore, each microsphere is equivalent to a separate, pre coated oneELISABoard; Compared to traditionalELISAThe sensitivity of fluorescence signal color development in technology is higher than that of general chemiluminescence. In addition, the use of flow cytometry for high sensitivity and amplification of fluorescence signals requires lower sample concentration and shorter experimental time to detect indicators in unknown samples; Additionally, conventionalELISAOperation, only one indicator in one sample can be detected at a time, and the operation between each detection sub standard needs to be carried out separately, often resulting in the consumption of precious samples, such as immune regulatory cytokines in the blood of patientsIFN-γ、TNF-a,IL-2Wait, throughBD CBAThe system can simultaneously detect multiple indicators in a sample, which is beneficial for someELISAExperiments that technology cannot meet provide new detection methods.

BD FACS Human Inflammation Cytokine Kit CBA Cytokine Detection Kit Flow cytometry

BD CBAThe commonly used reagent kits are as follows: