Automatic enzyme-linked immunosorbent assay analyzer

Enzyme linked immunosorbent assay (ELISA) analyzer, also known as microplate detector, is a specialized instrument for enzyme-linked immunosorbent assay (ELISA). Enzyme linked immunosorbent assay (ELISA) analyzers are divided into two categories: semi-automatic and fully automatic. Their working principles are basically the same, and the core is a colorimeter, which uses colorimetric methods for analysis. Used in conjunction with adapted reagents for qualitative and quantitative analysis of analytes in biological samples.

The enzyme-linked immunosorbent assay analyzer is essentially a disguised photoelectric colorimeter or spectrophotometer, and its basic working principle, main structure, and photoelectric colorimeter are basically the same. The light waves emitted by the light source lamp are converted into a beam of monochromatic light through a filter or monochromator, and enter the specimen to be tested in a plastic microplate. Part of the monochromatic light is absorbed by the specimen, while the other part is transmitted through the specimen and irradiated onto the photodetector. The photodetector converts these light signals into corresponding electrical signals, which are processed by signal processing such as preamplifier, logarithmic amplifier, and analog-to-digital conversion before being sent to the microprocessor for data processing and calculation. Finally, the results are displayed on the monitor and printer. The microprocessor also controls the movement of the mechanical drive mechanism in the X and Y directions through the control circuit to move the microplate, thereby achieving automatic sample injection detection process. Some enzyme-linked immunosorbent assay (ELISA) analyzers use manually moving microplates for detection, eliminating the need for mechanical drive mechanisms and control circuits in the X and Y directions, making the instrument more compact and structurally simpler.

Common faults and solutions:

Fault 1: No response after startup. Check if the power cord is properly connected and if the fuse is blown.

Fault 2: The original absorbance is mostly negative. It should be checked whether the absorbance value of the blank hole is too high. The original absorbance value printed by the enzyme-linked immunosorbent assay (ELISA) is obtained by subtracting the blank. If the blank is too high, negative values may appear.

Fault 3: When using the quantitative function of the enzyme-linked immunosorbent assay (ELISA) reader, the curve exhibits abnormal morphology. It is possible that the customer used a linear/logarithmic curve pattern and also used a standard substance with a concentration of 0, which caused the machine to be unable to take the logarithm of 0, resulting in an abnormal curve shape.

Fault 4: During quantitative measurement, the concentration value in the printed report appears, indicating that it exceeds the curve range. Excessive standard absorbance may cause the curve to shift upwards, making it impossible to print many samples with low absorbance values on the report. To solve this problem, dilute the standard sample to shift the curve downwards.