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market@diateklab.com
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No. 22 Luou Road, Xituo District, Hudai Industrial Park, Binhu District, Wuxi City
Wuxi Huaweidelang Instrument Co., Ltd
market@diateklab.com
No. 22 Luou Road, Xituo District, Hudai Industrial Park, Binhu District, Wuxi City
According to their different functions, enzyme-linked immunosorbent assay (ELISA) analyzers can be divided into light absorption ELISA analyzers, fluorescence ELISA analyzers, chemiluminescence ELISA analyzers, and multifunctional ELISA analyzers.
1. Light absorption enzyme-linked immunosorbent assay (ELISA) reader
The light absorption enzyme-linked immunosorbent assay (ELISA) is used to detect the absorbance of visible and ultraviolet light. After a specific wavelength of light passes through the sample in the microplate, the light energy is absorbed, and the absorbed light energy is proportional to the concentration of the sample, which can be used for qualitative and quantitative detection. The detection technology of light absorption is mature, low-cost, and easy to operate, but the dynamic range is narrow, the sensitivity is relatively low, and the specificity is not strong. Generally, tungsten lamps and deuterium lamps are used as light sources for visible light and ultraviolet light, respectively. However, UV/visible enzyme-linked immunosorbent assay (ELISA) can switch between the two light sources to meet the needs of different measurement wavelengths.
2. Fluorescent ELISA reader
Fluorescent enzyme-linked immunosorbent assay (ELISA) is used for fluorescence detection. After exciting the grating to split the light, a specific wavelength of light is irradiated onto the sample calibrated by the fluorescent substance, which emits longer wavelength emission light that passes through the emission grating and reaches the detector. The intensity of fluorescence is proportional to the concentration of the sample. Fluorescence detection has high sensitivity, can detect in real-time, is easy to use, and has various detection modes. However, it is susceptible to external interference, and the excitation light and emission light can easily affect each other, interfering with the detection.
3. Chemiluminescence ELISA reader
Chemiluminescent enzyme-linked immunosorbent assay (chemiluminescent enzyme-linked immunosorbent assay (chemiluminescent enzyme-linked immunosorbent assay (chemiluminescent enzyme-linked immunosorbent assay (chemiluminescent enzyme-linked immunosorbent assay (chemiluminescent enzyme-linked immunosorbent assay)) is derived from the self luminescence in biochemical reactions and can be divided into two types: glow type and flash type. Glow type luminescence is long-lasting, stable, and can last for a period of time; The flashing type has a short emission time, fast changes, and weak stability, and requires the use of an automatic sampler to perform. The chemiluminescence enzyme-linked immunosorbent assay (chemiluminescent enzyme-linked immunosorbent assay) has very high sensitivity and a wide dynamic range.
4. Multi functional enzyme-linked immunosorbent assay (ELISA) reader
The multifunctional enzyme-linked immunosorbent assay (ELISA) reader is a collection of the three types of ELISA readers mentioned above, integrating two or three types of ELISA readers into one. It can simultaneously perform detection of light absorption, fluorescence, and chemiluminescence, with powerful functions and a wide range of applications. It can be used as a research platform, avoiding duplicate purchases and is the preferred choice for laboratories.
According to different filtering methods, enzyme-linked immunosorbent assay (ELISA) can be divided into filter ELISA and grating ELISA.
1. Filter type enzyme-linked immunosorbent assay (ELISA) reader
The filter type enzyme-linked immunosorbent assay (ELISA) uses a filter to select wavelengths. After the full spectrum of light emitted by the light source passes through the filter, most of it is filtered, leaving only the wavelengths allowed by the filter itself. Therefore, only specific wavelengths can be selected for detection.
2. Grating ELISA reader
The grating enzyme-linked immunosorbent assay (ELISA) uses a grating for spectral analysis. The full spectrum of light emitted by the light source passes through the grating and is then separated by a series of slits distributed on the grating to obtain light of any wavelength, which is continuously adjustable. The grating enzyme-linked immunosorbent assay (ELISA) is convenient and flexible to use. It can select light of any wavelength and perform full wavelength scanning. Through full wavelength scanning, the absorption peak of unknown samples can be obtained, thus achieving the purpose of detecting unknown samples.