Antigen repair pot laboratory antigen repair pot

Only used for animal experimental research, etc

Antigen repair potAntigen Retriever

The success or failure of immunohistochemical methods depends on the preservation and exposure of antigenic determinants.Fix the organization with formaldehyde.The aldehyde group of formaldehyde and

Amino cross-linking of antigen proteins,Change the conformation of the determinant cluster.Thereby affecting the expression of detection proteins.To fully expose the antigenic determinants

Commonly used in antigen repair pots. Highly recommendedEMSA competitive product suitable for formaldehyde fixed tissue, wrapped in stone wax

Pre immunostaining treatment after burial, combined with special antigen repair buffer to ensure you receive the best quality immunostaining effectAntigen repair pot laboratory antigen repair pot

Fruit.

Antigen repair pot laboratory antigen repair pot

·It can be done once6Operate in different buffer solutions for antigen exposure

·Expose only antigenic determinants without damaging tissue morphology

·The experimental results are stable and have high reproducibility

·The series of glass slides are processed in an independent environment

How does the antigen repair pot work?

It seems that the antigen repair pot is like the pressure cooker we cook. This is a feature *“pressure cooker”, mainly used for antigen exposure

The main working principle of the work is that several compartments for glass slides can reach high temperatures(>120°C)High voltage. Sensitive sensor control

The device is used to maintain a certain temperature, pressure, and time, and these working parameters are obtained through rigorous scientific experiments

According to. When the desired temperature is reached, the antigen repair pot will remain at that temperature for a few minutes, after which the glass slides inside the pot will be cooled2How small is it

At that time, the special material of the inner pot body controlled the cooling speed of the pot and glass slide.

Who is the beneficiary?

The laboratory that studies pathology requires high-quality stained photos for publication

In a laboratory lacking technical personnel, both students and postdoctoral fellows can process glass slides for tissue staining without requiring a lot of time to explore

A small pathology laboratory that processes a limited number of slides per day

Samples that are expensive and rare, such as tissue chips or tissue samples like *, can be easily and reliably repaired using antigen repair pots

How to choose a buffer solution（Buffer）？

If you already know which buffer is used for microwave tissue antigen repair, then when using an antigen repair pot, this buffer

There is a great chance that liquid can also be used simultaneously. We offer four ready to use buffer solutionsR-Buffer A/B/C/UAt the same time, it also provides the same type of service

Gentle tissue processing buffer, which we callGSeries,R-Buffer AG/BG/CG/UG.

choiceBufferMainly consider the following two points:

1. The natural characteristics of antigens and the selected antibodies

The characteristics of the antigen itself and the location of the antigen are the primary considerations. We suggest that you choose the most suitable antigen expression environment at the beginning

suitBufferHere are some experiences for everyone's reference:

Most of the nuclear antigens (apoptosis-related, survival-related, proliferation-r

elated) R: Buffer A (or AG).

Cell adhesion molecules, cell membrane antigens (extracellular domain) R: Buffer A (or AG)

Cytoskeleton and cytoskeleton-associated molecules – R:Buffer A (or AG)

Intracellular domain of some adhesion molecules and surface receptors – R:Buffer B (or BG)

Intracellular domain of some adhesion molecules and surface receptors – R:Buffer C (or CG)

Most of antibodies raised against a linear peptide – R: Buffer U (or UG)

2. Fixed liquid and degree of fixation

The recommended antigen repair treatment for tissue slices is a type of preparation for normal adapted state slices. If the tissue block used for slicing is not available

Fully fixed, or overly fixed (left in formalin solution for too long), or using other fixatives, then

The process plan for organizing slices needs to be revised, and the most convenient way is to choose to use itGSeries buffer solution. For fixed

Materials are generally recommended for useU bufferOr in the same placeBufferAdd loops inside

Technical parameters of antigen repair pot:

Example of staining effect using antigen repair pot

1.Fixed in formalin, embedded in paraffin, sliced from human sigmoid colon tissue, and exposed to antigens using an antigen repair pot,R-

Buffer C (pH 6.0), tissue slides cooled overnight, antibodiesMIB-1, antigenKi-67，R-Detect HRPSystem staining

2. .Fixed in formalin, embedded in paraffin, sliced from human sigmoid colon tissue, and exposed to antigens using an antigen repair pot,R-

Buffer A (pH 8.1), tissue slides cooled overnight, antibodies#4B11, antigenCD8 (cell membrane)，R-

Detect HRPSystem staining.

Selection of instruments and accessories:

Selection of buffer solution: