ANTHOCYANIDIN ELISA Kit for Plant Anthocyanins

product nameANTHOCYANIDIN ELISA Kit for Plant Anthocyanins

Preparation before Elisa's experiment

1. Carefully read the instruction manual

2. Confirm that the reagent kit is within its validity period

3. Confirm that all reagents are complete (quantity, volume) according to the instructions

4. Specimen preparation should be standardized, and each specimen should be prepared (stored) in a volume of 2-3 or more wells, and should be packaged as backup as much as possible. Unable to conduct experiments in the short term, pay attention to low-temperature storage

Prepare all necessary additional items for the experiment, such as test tubes, pipettes, pipettes, centrifuges, etc

Determine the required amount of reagents based on the number of test specimens

According to the instructions, balance the reagents used to room temperature and prepare the required reagents

DIY sandwich ELISA common technical guide

When selecting antibodies, choose a monoclonal antibody and a multi antibody for pairing; If two monoclonal antibodies are selected, they must recognize antibodies with different epitopes; Select antibody pairs from the same company.

In the 9ELISA experiment, in order to determine the * signal and the lowest background, the capture antibody (0.5-4 μ g/ml) and the detection antibody (0.25-2 μ g/ml) should be titrated against each other in the pre experiment. At the same time, a series of dilutions of standard samples should be included within an appropriate range. Operate according to the range provided in the reagent manual.

Preparation of 10 standard samples: For each cytokine, carefully read the instructions and pay attention to the details of each batch. Before using cytokines, centrifuge the reagent bottle instantaneously to retrieve as many cytokines as possible. According to the details in each batch of instructions, dissolve the freeze-dried cytokines.

The linear range of the standard curve of the antigen to be tested can be obtained by diluting the standard sample 8 times in a 2-fold series from 2000pg/ml to 15pg/ml. The sensitivity can be improved within a certain range by using standard ELISA operating procedures, amplification kits, third class reagents, or changing the enzyme substrate system.

To optimize sensitivity, it is recommended to incubate standard samples and specimens overnight.

If peroxidase is used as a colorimetric system, it is strictly prohibited to add sodium azide to the washing solution and diluent. Sodium azide can inhibit the activity of peroxidase.

When measuring antigens in mixed liquids, such as serum, it is recommended to add unrelated Igs to the sample diluent

ELISA is a new technology in immunodiagnosis, which has been successfully applied to immunodiagnosis of infectious diseases, parasitic diseases, and non infectious diseases caused by various pathogenic microorganisms. It has also been applied to the quantitative determination of large molecule antigens and small molecule antigens. Based on the results of its use, ELISA is considered to be sensitive, specific, simple, rapid, stable, and easy to automate. Not only applicable for the examination of clinical specimens, but also suitable for serum epidemiological investigations as hundreds or even thousands of specimens can be examined within a day. This method can not only be used to determine antibodies, but also to determine circulating antigens in body fluids, making it a good method for early diagnosis.

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ANTHOCYANIDIN ELISA Kit for Plant Anthocyanins