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No.1 Fengcheng 3rd Road East Section, Xi'an Economic and Technological Development Zone, Shaanxi Province
Shaanxi Xinyan Bomei Biotechnology Co., Ltd
No.1 Fengcheng 3rd Road East Section, Xi'an Economic and Technological Development Zone, Shaanxi Province
chemical property:
Reactivity: NHS ester groups can covalently bind with primary amines on biomolecules such as proteins, antibodies, nucleic acids, etc., forming stable amide bonds.
Stability: It has high thermal stability and photochemical stability, suitable for single-molecule detection and high-resolution microscopy.
Structural formula:

Overview of Basic Knowledge:
670269-33-7,ATTO 590 Succinimidyl Ester
Chinese name: ATTO 590 NHS activated ester
English name: ATTO 590 Succinimidyl Ester, ATTO 590 NHS ester
CAS:670269-33-7
Molecular formula: C41H42ClN3O11
Molecular weight: 788.24
Specification standards: 1mg, 5mg, 10mg
Reagent manufacturer: Shaanxi Xinyan Bomei Biotechnology Co., Ltd
Related applications:
Fluorescent labeling: used to label biomolecules such as proteins, antibodies, nucleic acids, etc., suitable for fluorescence microscopy, flow cytometry, and fluorescence imaging.
Single molecule detection: suitable for single molecule detection and high-resolution microscopy techniques such as PALM, dSTORM, and STED.
Fluorescence Resonance Energy Transfer (FRET): Used in conjunction with BHQ-2 dye, it can be used for FRET applications and molecular beacon experiments.
Usage:
1. Mark reaction conditions:
Reaction pH: It is recommended to label between pH 8.0-9.0.
Reaction temperature: room temperature.
Reaction time: usually 1-18 hours.
2. Storage conditions: Store in the dark at -20 ° C to avoid repeated freezing and thawing.
3. Post labeling purification: The labeled product can be filtered through gel (such as Sephadex G-25) to remove the unconjugated dye.
Related reagents (partial):
Atto 647N alkyne
ATTO 532 hydrazide
ATTO 740 carboxy
ATTO 740 biotin
ATTO 633 iodacetamide
Atto 700 NHS-ester
Atto Rho6G NHS ester
Atto 655-maleimide
Atto 390 azide
Atto 565 maleimide
Atto Rho6G-Biotin
Acto 647N DPPE
Atto 633 DOPE
Atto 425-Biotin
Phalloidin–Atto 594
Phalloidin–Atto 740
Atto 565-Streptavidin
670269-33-7,ATTO 590 Succinimidyl Ester