173 degree online dynamic laser particle size

1、 PN3700 Zetasizer Nano173 degree online dynamic laser particle size

High sensitivity online dynamic light scattering detector, customized for on-site flow fractionation (AF2000, CF2000, and TF2000) for connection use. Online data linking can be performed to obtain the particle size (Rh), molecular weight (Mw), and corresponding distribution of dispersed particles and molecules through measurement. These dispersed particles and molecules are separated and fractionated through flow, centrifugation, and thermal field flow.

II173 degree online dynamic laser particle sizeintroduction

1. Zetasizer flow detector

The advanced detector hardware allows it to operate in online mode, which is connected to FFF and has a dedicated flow pool for continuous DLS, or in offline mode, using a standard reaction cup pool as an independent detector for batch DLS measurements. The system allows up to two additional input external FFF detectors (such as RI, UV, FLD, etc.) and PN9020 interface box for remote start function when used together with automatic operation. The Zetasizer Flow-S connection is the last detector online. The switch between flow and batch mode measurement is achieved by simply replacing the reaction cup within a few seconds. The system will automatically identify elution FFF peaks without any calibration, expressed in size or molecular weight. The software allows coverage of RI, UV, and FLD tracking of Rh data and static light scattering intensity signals. Using Zetasizer Flow-S no longer requires relying on FFF size or molecular weight calibration standards.

2. Zetasizer flow software

Advanced detector software allows for automated operations and is easy to use with repetitive SOPs between operators, systems, and the site. There is a custom report generator integrated to meet the requirements of each laboratory. Multiple parameters can be easily monitored, such as temperature trend analysis, time trend analysis, selected parameter trend analysis, direct comparison, and over drawing of full range statistical chart results.

3. Online Mode Description

Particle size range (Dh): 2.0 nm-2.0 μ m;

Molecular weight range estimated based on Dh or calculated based on Debye diagram: 10E4-10E7 Da;

Sensitivity: 67.000 Da BSA total sample 200 μ g; 50 μ g 60nm polystyrene latex, typical FFF flow rate range: 0.05 to 1.0 mL/min;

Pressure range: The maximum pressure difference at the inlet/outlet of the reaction cup is 1.5 bar;

Flow cell volume: 480 μ L;

Depending on the sample material, concentration, and FFF flow rate;

4. Batch processing mode specification

Particle size range (Dh): 0.3nm-10 μ m; Peak mode range (Dh), 0.6 nm-8.9 μ m;

Molecular weight range estimated based on Dh: 342-2x10E7 Da;

Molecular weight range calculated by Debye diagram: 980-2x10E7 Da;

Sensitivity: 0.1 ppm *, 0.1 mg/mL, 15 kDa protein; 0.1 ppm 60 nm polystyrene latex,Concentration. Maximum value: 40% w/v;

Minimum sample volume: 12 μ L;

Depending on the sample material, concentration, and FFF flow rateOptions (to be selected);

High power laser/alternative wavelength 50MW, 532nm, high temperature up to 120 ° C, 633nm or 532nm narrowband filter to improve the signal of fluorescent samples;

Pharma 21 CFR Part 11 Software Options, enabling research software options that contribute to ER/ES compliance and advanced system utilities;

5. Zetatentorial measurement optionsRequired parts for computers with the following low recommended computer specifications:

Pentium P4 PC 2.8 GHz dual core processor, 2GB RAM, 160 GB hard drive, 1024 x 768 screen resolution, CD-ROM drive, USB port, Windows XP Pro (SP3), Vista or Win7 operating system. Any used laptop must have a free USB port to run

PN9020 interface box is required for the automatic operation of FFF and Zetasizer flow in the system, and additional flow sensors must be ordered.